SHCBP1在肝细胞癌中的表达及其临床意义  

Expression of SHCBP1 in Hepatocellular Carcinoma and Its Clinical Significance

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作  者:陶汉川 顾澄宇[1] 蔡兵[1] 

机构地区:[1]南京医科大学附属无锡市人民医院肝胆外科,214023

出  处:《医学研究杂志》2013年第11期109-113,共5页Journal of Medical Research

摘  要:目的研究SHCBP1基因在人肝癌组织中的表达情况,探讨SHCBP1基因在肝细胞癌发生发展中的作用。方法采用半定量RT-PCR、荧光定量PCR(时实PCR)、免疫组织化学法等方法检测SHCBP1基因在52例临床肝细胞癌样本的癌及癌旁组织和人正常组织的表达情况,应用统计学方法分析结果。结果①SHCBP1基因mRNA表达水平在人正常组织中表达具有明显差异;在肝细胞癌样本中明显高于癌旁组织(P<0.05);②免疫组化结果检测显示SHCBP1蛋白在癌旁组织组织中低表达或不表达,而在肝细胞癌组织中表达明显增高,且棕色染色区域主要集中在肿瘤细胞边缘细胞膜位置;③Western blot检测结果显示SHCBP1蛋白在肝细胞癌样本中高表达,癌旁组织中不表达或低表达(P<0.05);④临床统计资料分析显示SHCBP1基因的表达情况与肝细胞癌患者的性别、年龄、临床分期无关,而与肿瘤的直径大小、个数、分化程度、脉管浸润紧密相关。结论 SHCBP1基因在肝细胞癌组织中呈高表达,可能通过影响多种癌症信号通路分子促进肝癌的发生发展,同时该基因可能成为肝癌治疗的潜在新靶点。Abstract Objective To investigate the expression of SHCBP1 in hepatocellular carcinoma,and evaluate the role of SHCBP1 in the development and progression of HCC. Methods RT - PCR,real - time PCR,immunohistochemistry and were used to detect the expres- sion of SHCBP1 in 52 pairs of human HCC specimens and human normal tissue. The results were statistically evaluated. Results (~The expression of SHCBP1 mRNA level had evident individual differences among human normal tissue. Compared with the adjacent tissues, the expression level of SHCBPI in hepatocarcinoma tissue was significantly higher than that in non - HCC tissue and the mean time was 3.33 ( P 〈 0.05 ). ~)The immunohistochemical staining showed that the expression of SHCBP1 was obviously up - regulated in HCC, and down - regulated or absence in non - HCC tissue. The dyed mainly accumulated in the membrane of HCC cells. (~)Western blot results showed that SHCBP1 protein was highly expressed in HCC tissues. In contrast, the no or low expression was detected in paracancerous tissues;(~ The clinical data showed that the expression of SHCBP1 had no correlation with gender, age, stage; but it was positively associated with tumor size, number, vascular invasion and metastasis, the differentiation degree (P 〈 0.05 ). Conclusion SHCBPI gene is up - regula- ted in HCC tissue. It may play important roles in activation of intracellular signal transduction pathway. Study of SHCBPI gene will pro- vide new therapeutic target for targeted therapy of Hepatocarcinoma.

关 键 词:SHCBP1基因 原发性肝癌 Src同源区2结构域蛋白C(Shc) 

分 类 号:R735.7[医药卫生—肿瘤]

 

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