酪蛋白激酶2催化亚单位对雄激素受体信号转运表达的参与作用  

Both CK2 catalytic subunits involves in androgen receptor signaling in prostate cancer cells

作　　者：尧凯[1] 高晓燕[2] 黄必军[2] 李再尚[1] 周芳坚[1] 李本义 韩辉[1] 

机构地区：[1]中山大学肿瘤防治中心泌尿外科,广州510060 [2]华南肿瘤学国家重点实验室 [3]Department of Urology, The University of Kansas Medical Center, Kansas City, USA

出　　处：《中华医学杂志》2013年第42期3360-3363,共4页National Medical Journal of China

基　　金：国家自然科学基金（81302224）

摘　　要：目的 探讨酪蛋白激酶2（CK2）催化亚单位是否都参与前列腺癌细胞雄激素受体信号转运表达.方法 CK2选择性抑制剂四溴肉桂酸（TBCA）和基因特异性小干扰RNA（siRNA）应用到多种前列腺癌细胞株进行检测,探讨CK2不同催化亚单位在雄激素受体（AR）信号传导的作用.萤光素酶基因报道分析检测雄激素受体反式激活,免疫荧光方法检测AR核定位以及定量聚合酶链反应（PCR）检测AR介导的基因表达.结果 TBCA阻断AR核转位和基因表达（P〈0.01）.雄激素反应元件荧光素酶基因检测结果显示CK2α和CK2α＇siRNA都显著减弱R1881（人工合成的雄性激素） 刺激引发的前列腺癌细胞活性（38.5与31.4）,2种CK2催化亚单位都参与雄激素刺激的核转位和AR介导的基因表达（P〈0.05）.结论 CK2亚单位CK2α和CK2α＇都参与了雄激素受体信号转运表达.Objective To explore the roles of different casein kinase 2 （CK2） catalytic subunits in androgen receptor （AR） signaling in prostate cancer cell lines. Methods Prostate cancer cell lines were maintained. Immunofluorescent staining was performed to determine AR nuclear translocation in PC-3/AR cells with R1881 pretreatment and luciferase gene reporter assay used to determine AR transactivation in LNCaP cells. And reverse transcription-polymerase chain reaction （RT-PCR） was employed to evaluate the mRNA level of prostate-specific antigen （ PSA ）. Results R1881-induced AR nuclear localization was reduced significantly （P 〈0.01 ）. R1881-stimulated ARE-LUC reporter activity in LNCaP cells decreased with reduced level of PSA mRNA, an AR endogenous target （P 〈 0. 05 ）. To confirm the target specificity, the gene-specific siRNAs were used for both CK2ct and CK2α＇ subunits. The results of ARE-LUC assay （38. 5 vs 31.4） suggested that both CK2 catalytic subunits were involved in androgen-stimulated AR nuclear translocation and AR-mediated gene expression in prostate cancer cells. Conclusion Both CK2 catalytic subunits are involved in androgen receptor signaling of prostate cancer cells.

关 键 词：酪蛋白激酶2 前列腺肿瘤 雄激素受体 四溴肉桂酸 

分 类 号：R737.25[医药卫生—肿瘤]