针叶树基因组特征及其序列资源挖掘进展  

Characteristics of Conifer Genome and Recent Advances in Conifer Sequence Resources Mining

作　　者：许晨璐[1] 孙晓梅[1] 张守攻[1] 

机构地区：[1]中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京100091

出　　处：《植物学报》2013年第6期684-693,共10页Chinese Bulletin of Botany

基　　金："十二五"国家科技支撑计划(No.2012BAD01B01)

摘　　要：针叶树是裸子植物中最大也是分布最广的一支。作为多年生木本植物,针叶树不仅为工业提供建筑、造纸等重要原料和其它可再生能源,而且在北半球的生态平衡中也起着重要作用。因其独特的分类地位、重要的经济价值和生态价值,针叶树序列资源挖掘备受重视。然而其庞大且复杂的基因组阻碍了这一进程,截至2013年4月,尚无获得全基因组序列的针叶树种。随着第2代测序技术的出现及生物信息学的快速发展,针叶树序列资源挖掘也从转录组过渡到全基因组测序,后者已在松属(Pinus)、云杉属(Picea)和黄杉属(Pseudotsuga)部分树种中开展。该文首次归纳了针叶树基因组特征,回顾了针叶树序列资源挖掘进程,并重点介绍了火炬松(Pinus taeda)、欧洲云杉(Picea abies)和白云杉(Picea glauca)的全基因组测序项目。Conifers are the largest and most ubiquitous group of gymnosperms. They are woody perennials that shape many northern hemisphere ecosystems and support large industries through the provision of wood, fiber, and energy. Sequencing conifer genomes is relevant because of their taxonomic position, ecological significance, and economic im- portance. However, the large and complex genomes of conifers have hindered the mining process, and by April 2013, no whole-genome sequences for conifer have been obtained. With the emergence of next-generation sequencing and rapid development of bioinformatics, the conifer sequence resources mining project has transitioned from the transcriptome to whole-genome sequencing projects, initiated in Pinus, Picea and Pseudotsuga genus. This review summarizes the characteristics of the conifer genome, reviews the currant status of genome sequencing of conifers and outlines the cur- rent state of knowledge concerning the genomes of conifer （Pinus taeda, Picea abies and Picea glauca）.

关 键 词：针叶树 基因组 研究进展 转录组 全基因组测序 

分 类 号：S791[农业科学—林木遗传育种]