复方青黛颗粒对溃疡性结肠炎模型大鼠结肠MMP-1及TIMP-1表达的影响  

Effects of Compound Qingdai Granules on the expression of MMP-1and TIMP-1of model rats with ulcerative colitis

作　　者：王希[1] 杜立阳[1] 张艳[1] 梁庆昱 

机构地区：[1]中国医科大学第一附属医院中医科,辽宁沈阳110016

出　　处：《中国中西医结合消化杂志》2013年第11期565-568,共4页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金：辽宁省自然科学基金资助项目(No.20092130)

摘　　要：[目的]观察复方青黛颗粒对溃疡性结肠炎(UC)模型大鼠结肠基质金属蛋白酶1(MMP-1)及其特异性组织抑制因子(TIMP-1)基因表达的影响,探讨其治疗UC的作用机制。[方法]用三硝基苯磺酸(TNBS)制备UC大鼠模型,将52只SD大鼠随机分为正常对照(空白)组、模型组、美沙拉嗪(5-ASA)组,复方青黛颗粒低、中、高剂量组,从造模后第3天开始分别每天灌胃给药1次至实验结束,第14天(灌胃10d后),用逆转录聚合酶链反应(RT-PCR)法检测MMP-1、TIMP-1基因表达的水平。[结果]模型组MMP-1、TIMP-1基因相对表达量均明显高于空白组(P<0.05);复方青黛颗粒中、高剂量组MMP-1相对表达量及中、高剂量组和5-ASA组TIMP-1相对表达量均明显低于模型组(均P<0.05)。[结论]复方青黛颗粒能有效治疗TNBS诱导的UC模型大鼠,可能与复方青黛颗粒抑制MMP-1、TIMP-1基因表达有关。[Objective]To investigate the effects of Qingdai Granules（QDG）on the expression of MMP-1,TIMP-1 mRNAs in the colonic mucosa of rats with ulcerative colitis.[Methods] Ulcerative colitis was induced in rats by giving an enema of trinitrobenzene sulphonic acid（TNBS） and ethanol.Fifty two experimental animals were randomly divided into six groups：normal group,model group,Mesalazine （5-ASA） group（500 mg/kg）,and low,medium and high-dose QDG groups（600,900 and 1 200 mg / kg）.Except the normal group,the other groups were given intragastric administration of normal saline,5-ASA,and different concentrations of QDG,respectively,during the 3rd ～ 12th days after model was established.Thereafter,rats were killed and the expression of MMP-1 and TIMP-1mRNAs in the colon of rats was detected by reverse transcription polymerase chain reaction（RT PCR）.[Results] Compared with the normal group,the expression of MMP-1 and TIMP-1mRNAs in the colon of rats in the model group was significantly up-regulated.Compared with the model group,the expression of MMP-1 mRNA in the colon of rats in the medium and high-dose QDG groups was significantly down-regulated and the expression of TIMP-1 mRNA in the high-dose QDG group was significantly down-regulated.[Conclusion] QDG can exert protective effects against rat ulcerative colitis perhaps by down-regulating MMP-1 and TIMP-1mRNAs expression.

关 键 词：结肠炎 溃疡性 复方青黛颗粒 基质金属蛋白酶-1 特异性组织抑制因子-1 

分 类 号：R574.62[医药卫生—消化系统] R282.71[医药卫生—内科学]