呼吸道内具核梭杆菌与阻塞性肺疾病相关性研究  

Detection and study of Fusobacterium nucleatum in the acute exacerbation of chronic obstructive pulmonary disease

作　　者：谭丽思[1] 王宏岩[1] 赵海礁[1] 潘亚萍[1] 康健[2] 

机构地区：[1]中国医科大学口腔医学院牙周科,沈阳110002 [2]中国医科大学附属第一医院呼吸科,沈阳110001

出　　处：《中国实用口腔科杂志》2013年第11期664-667,共4页Chinese Journal of Practical Stomatology

基　　金："十一五"国家科技支撑计划(2007BAI18B02)

摘　　要：目的检测慢性阻塞性肺疾病急性加重期(AE-COPD)患者呼吸道内具核梭杆菌(Fn.)的定植,探讨Fn.与AE-COPD间的关系。方法选择2008年10月至2009年4月中国医科大学附属第一医院、盛京医院确诊为AE-COPD的患者53例,采集呼吸道分泌物,提取细菌DNA,应用SYBR@GreenⅠPremix Ex Taq TM模式的实时PCR技术,对Fn.进行定量检测,并计算其所占总菌的比例。同时记录患者FEV1占预计值比例(%)及口腔牙周指标。结果 53例样本中有32例检出Fn.,Fn.检出率为60.38%,占总菌的比例为(44.58±16.47)%。Fn.相对含量与FEV1占预计值比例呈负相关关系(P<0.05)。Fn.相对含量与简化口腔卫生指数及附着丧失水平呈正相关关系(P<0.05);与牙龈出血指数、探诊深度、缺失牙数无相关性(P>0.05)。结论 AE-COPD患者呼吸道内存在Fn.,Fn.相对含量随着肺功能的减弱而增加;Fn.与AE-COPD密切相关。Objective To detect the presence of Fusobacterium nucleatum （Fn.） in the acute exacerbation of chronic ob- structive pulmonary disease（AE-COPD）and to investigate its relationship with FEV1 and periodontal index. Methods The sputum samples of AE-COPD were collected and were extracted DNA of bacterium. The amount of Fn. and the to- tal number of bacterial cells were determined by real-time PCR based on SYBR Green 1 Premix Ex TaqTM. FEV1 and periodontal parameters of each AE-COPI） were recorded, and then investigate the relationship between Fn. and FEV1 and periodontal index. The data were statistically analyzed using SPSS17.0 software. Results The detection rate of Fn. was 60.38% and the relative quantity of Fn. was （44.58＋ 16.47）%. There was negative correlation between the relative quantity of Fn. and FEV1 （P 〈 0.05）. There was positive correlation between the relative quantity of Fn. and simplified oral hygiene index and clinical attachment loss（P 〈 0.05）, Conclusion Fn. can colonize in the sputum samples in the AE-COPD and colonization of Fn. is relevant to FEV1 and periodontal parameters.

关 键 词：具核梭杆菌 慢性阻塞性肺疾病急性加重 牙周炎 

分 类 号：R78[医药卫生—口腔医学]