红藻氨酸致痫大鼠外周血炎性因子与癫痫发作程度的相关性研究  

Correlation between peripheral blood inflammatory cytokines and kainic acid-induced seizure severity inrats

作　　者：冯小明[1] 马建霞 罗晓红[1] 罗建军[1] 陆璐[1] 牛廷献[1] 

机构地区：[1]兰州军区兰州总医院动物实验科,兰州730050 [2]兰州石化总医院药剂科

出　　处：《中华行为医学与脑科学杂志》2013年第11期969-971,共3页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：基金项目：甘肃省自然科学基金（1107RJZA269）

摘　　要：目的探讨红藻氨酸（kainicacid，KA）致痫大鼠外周血炎性因子的水平与癫痫严重程度的关系。方法140只Wistar大鼠随机分为对照组和模型组，每组70只。模型组以10mg／kgKA腹腔注射建立模型，对照组以生理盐水同样处理。观察行为学变化。以ELISA方法检测不同时点血清TNF-α、IL-1β、IL-4和IL-10的表达水平。结果KA注射后，大鼠在3h-9h进入癫痫大发作期。与对照组相比[6h：（12．3±3．1）pg／ml，12hl（11．5±3．8）pg／ml]，模型组在6h-12h促炎因子TNF—α[6h：（21．5±3．2）pg／ml，12h：（20．6±4．2）pg／ml]和IL-1β的水平明显升高（P〈0．05）；抗炎因子IL-4只在12h时明显升高[（53．55±3．08）pg／ml，（33．26±4．16）pg／m1]（P〈0．05），IL-10水平差异无统计学意义（P〉0．05）。结论促炎因子TNF-α和IL-1β参与了癫痫的发作过程，其水平与癫痫发作程度密切相关；抗炎因子IL-4和IL-10水平的变化与癫痫的发作程度无显著相关性。Objectives To investigate the correlation between the level of peripheral blood inflammatory cytokines and kainic acid-induced seizure severity in rats. Methods 140 rats were divided into control and model group randomly,70 rats in each group. Model group rats were injected with kainic acid （ 10 mg/kg） by intraperito- heal, and the control rats were injected with sodium chloride. The change of their behaviors was observed and the concentrations of TNF-α, IL-1 β, IL-4 and IL-10 were determined by ELISA in each group at different times.Results The rats showed epilepsy grand mal in 3 h-9 h after KA injection. The concentrations of TNF-α and IL-1β in mod- el groups were significantly higher than those in control group in 6 h-12 h（6 h： （21.5±3.2） pg/ml vs （12.3±3.1） pg/ml; 12 h： （20.6±4.2）pg/ml vs （11.5±3.8）pg/ml） （P〈0.05） and IL-4 in model group was higher at only 12 h （（53.55±3.08） pg/ml vs （33.26±4.16）pg/ml） （P〈0.05）. The level of IL-10 in model groups was not statistically significant compared with control group （P〉 0.05）. Conclusion The proinflammatory cytokines （TNF-α and IL- 1β） participate in the seizure procedure, meanwhile their levels and seizure severity have eminent correlations, but antiinflammatory cytokines （IL-4 and IL-10） had not.

关 键 词：癫痫 红藻氨酸 发作程度 炎性因子 

分 类 号：R742.1[医药卫生—神经病学与精神病学]