JC病毒大T抗原及β-catenin在结直肠腺癌中的表达及意义  

Expression and Clinical Significance of JCV T-Ag and β-catenin Proteins in colorectal adenocarcinoma

作　　者：朱小宝[1] 田小林[1] 宋永攀[1] 庞凌坤[1] 

机构地区：[1]桂林医学院附属医院胃肠外科,广西桂林541001

出　　处：《结直肠肛门外科》2013年第4期206-210,共5页Journal of Colorectal & Anal Surgery

基　　金：广西壮族自治区卫生科研基金资助项目(Z2009042)

摘　　要：目的探讨JC病毒(JCV)早期基因编码产物大T抗原(large tumor antigen,T-Ag)的表达及β-catenin(β-连接素)蛋白表达与结直肠腺癌发生发展的关系。方法应用免疫组化法检测65例结直肠腺癌和20例非肿瘤胃黏膜中JCV T-Ag、β-catenin蛋白的表达。结果 65例结直肠腺癌中JCV T-Ag阳性表达率为38.4%(25/65)。20例非肿瘤结直肠黏膜中无JCV T-Ag蛋白阳性表达。结直肠腺癌中JCV T-Ag阳性表达与分化程度、浸润深度无统计学意义(P>0.05)。与淋巴结转移间有统计学意义(P<0.05),β-catenin蛋白在65例结直肠腺癌中正常表达率为47.7%(31/65),65例结直肠腺癌中弱阳性表达或不表达为52.3%(34/65)。结直肠腺癌中β-catenin蛋白异常表达与分化程度、浸润深度、淋巴结转移之间差异具有统计学意义(P<0.05),在20例非肿瘤胃黏膜100%阳性表达,即JCV T-Ag与β-catenin蛋白在结直肠腺癌组织中的表达呈负相关(P<0.01)。结论 JCV T-Ag早期基因编码产物大T抗原与β-catenin蛋白在结直肠腺癌的发生、发展中起重要作用。Objective To explore the role of JC virus （JCV） early region gene encoding product large tumor an- tigen（ T-Ag ） and β-eatenin proteins expressions in colorectal adenocareinoma occurrence and development. Meth- ods The expressions of JCV T-Ag and β-eatenin proteins were detected by immunohistochemistry in 20 cases of nor- mal colorectal mucosa and 65 cases of colorectal cancer tissues. Results The positive rate of JCV T-Ag of colorectal adenocarcinoma was 38.4% （ 25/65 ） in 65 cases , and none of the JCV T-Ag was detected in the normal colorectal mucosa in 20cases. The positive expression of JCV T-Ag in the colorectal adenocarcinoma has no direct relationship to the differentiation or depth of infiltration （ P 〉 0. 05 ）. But it was colsely correlated with lymph node metastasis （ P 〈0. 05 ）. The positive rate of β-catenin of colorectal adenocarcinoma was 47.7% （31/65）. The Weakly positive and negative rate（ Abnormal expression） was 52. 3% （ 34/65 ） in 65 eases. The Abnormal expression of theβ-cate- nin was closely correlated with lymph node metastasis and differentiation or depth of infiltration. The normal expres- sion of β-catenin was detected in all normal colorectal mucosa in 20 cases. Conclusion JCV T-Ag early region gene encoding product large tumor antigen and β-catenin proteins were over-expressed in colorectal adenocarcinoma and their expressions had relevance to the carcinogenesis and the, progression of colorectal adenocarcinoma.

关 键 词：结直肠腺癌 JC病毒 大T抗原 Β-连接素 免疫组织化学 

分 类 号：R735.3[医药卫生—肿瘤]