抗菌肽对热应激肉鸡血液学及血氧自由基的影响  

Effects of antibacterial peptides on hematology indexes and oxygen free radicals of broilers under heat stress condition

作　　者：包汇慧[1,2] 高贤彪[2] 佘锐萍[2] 贾旭东[1] 陈建[2] 肖鹏[2] 常玲玲[2] 汪会玲[1] 支媛[1] 耿雪[1] 夏抗抗 

机构地区：[1]国家食品安全风险评估中心卫生部食品安全风险评估重点实验室,北京100021 [2]中国农业大学动物医学院,北京100193 [3]北京市丰台区动物卫生监督所,北京100071

出　　处：《中国食品卫生杂志》2013年第6期489-494,共6页Chinese Journal of Food Hygiene

基　　金：国家自然科学基金项目(30471301)

摘　　要：目的了解猪小肠抗菌肽在热应激条件下对肉鸡血液学及血氧自由基的影响。方法选用30只两周龄健康华都AA+肉鸡,称重后随机分为3组:常温对照组、单纯热应激组和抗菌肽+热应激组,每组10只,热应激10天。结果抗菌肽+热应激组与单纯热应激组比较全血黏度、血浆黏度、红细胞压积明显降低(P<0.01);抗菌肽+热应激组血清中总蛋白(TP)、球蛋白(GLO)、葡萄糖(GLU)及谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)与单纯热应激组比较均升高;总胆固醇(TC)、甘油三酯(TG)、尿素(UREA)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)与单纯热应激组比较明显降低;抗菌肽+热应激组SOD、GSH-Px活性与单纯热应激组比较明显升高(P<0.01);MDA含量与单纯热应激组比较差异有统计学意义(P<0.01)。结论热应激条件下口服抗菌肽可以调节肉鸡血液成份及流变状态、增强体液免疫、平衡氧自由基、降低氧化应激反应,使机体有效抵抗热应激。Objective To explore the effect of porcine intestinal antimicrobial peptides （AMP） on hematology indexes and oxygen free radicals of broiler chicken under heat stress condition. Methods 30 10-day-old Arbor Acre broiler chickens were randomly allocated to three groups with 10 birds per group. The groups were control group （normal room temperature） , heat stress group （simulate summer high temperature） , and AMP treated group （2 mg per bird） under heat stress condition. The chickens were treated with high temperature for 10 days, 8 hours per day. Serum was collected after ten days. Results The results showed that whole blood viscosity, plasma viscosity, and packed cell volume of AMP treated group under heat stress condition had significant difference compared to heat stress group （P 〈 0.01 ） ; TP, GLO, GLU, ALT, AST and ALP of AMP treated group were significantly higher than those of heat stress group; TC, TG, UREA, LDL and HDL of AMP treated group were significantly lower than those of heat stress group. Comparison between AMP treated group and heat stress group revealed significantly increase of SOD and GSH-Px （P 〈 0.01 ） , and decrease of MDA （P 〈 0. 01 ）. Conclusion In conclusion, AMP could modulate blood components and flowing deformation, improve humoral immunity, balance oxygen free radicals to resist heat stress. Therefore, AMP has the potential as immunomodulator in broiler production.

关 键 词：抗菌肽 热应激 肉鸡 血液流变学 血生化 血氧自由基 组织病理 毒理实验 

分 类 号：TS251.1[轻工技术与工程—农产品加工及贮藏工程]