免疫印迹法测定乳腺肿瘤患者胸苷激酶  

Anti-TK1 antibody in detecting thymidine kinase 1 in serum of breast tumor patients

作　　者：李艳[1] 张平安[1] 邹力[2] 何其敏 

机构地区：[1]湖北医科大学附属第一医院检验科,武汉430060 [2]湖北医科大学附属第一医院普外科,武汉430060 [3]Karolinska institute

出　　处：《中华检验医学杂志》2000年第6期334-336,共3页Chinese Journal of Laboratory Medicine

摘　　要：目的　探讨用免疫印迹发光法 ,检测乳腺肿瘤患者血清细胞质胸苷激酶 (TK1)的研究及临床应用。方法　利用抗胸苷激酶单克隆抗体 (anti TK1mAb)建立点免疫印迹发光法 ,分析乳腺肿瘤患者、非肿瘤患者和体检健康者血清TK1的水平。结果　术前的乳腺癌患者、乳腺良性肿瘤患者、非肿瘤患者和体检健康者血清TK1的水平分别为 (39.3± 33.9) ,(3.7± 3.9) ,(1.7± 0 .5 )和 (1.4±0 .5 )pmol/L。淋巴结转移的术前和术后乳腺癌患者、乳腺良性肿瘤患者血清TK1与体检健康者差异有显著意义 (P <0 .0 0 1,P <0 .0 0 1,P <0 .0 5 ) ;而淋巴结未转移的术后乳腺癌患者、非肿瘤患者血清TK1与体检健康者差异无显著意义 (P >0 .10 ,P >0 .0 5 )。通过对术后乳腺癌患者血清TK1检测发现 ,淋巴结未转移患者TK1水平明显低于淋巴结转移患者 (P <0 .0 0 1)。结论　该方法有较好的敏感性与特异性 ,用于血清TK1的检测可作为乳腺癌的辅助诊断。Objective To evaluate the value of immunoblotting in the detection of serum thymidine kinase 1 (TK1) in breast tumor patients. Methods TK1 in sera from breast tumor patients, non tumor patients and healthy volunteers were determined with immunoblotting assay.Results The levels of TK1 in breast cancer patients before operation, patients with benign breast tumor, non tumor patients, and healthy volunteers were (39.3± 33.9), (3.7±3.9), (1.7±0.5) and (1.4±0.5) pmol/L respectively. The value of TK1 was higher in malignant and benign breast neoplasms patients than in healthy volunteers. However, no significant difference in the TK1 level was found between breast cancer patients without metastasis after operation, non tumor patients, and healthy volunteers.Conclusions The immunoblotting of TK1 by anti TK1 antibody is a sensitive and specific assay. It can be used to monitor and predict the efficiency of the therapy as well as the breast neoplasms progression during clinical course.

关 键 词：胸苷激酶 免疫印迹法 乳腺肿瘤 

分 类 号：R737.9[医药卫生—肿瘤] R730.43[医药卫生—临床医学]