俄歇电子发射体^(67)Ga-EDTMP对骨肉瘤细胞株辐射效应的形态学变化  

The Morphological Change of Human Osteosarcoma Cell Line (HOS-8603) Induced by Auger Electron Emitter ^(67) Ga-EDTMP

作　　者：许玉杰[1] 李晴暖[2] 朱然[1] 朱本兴[1] 张勇平[2] 张晓东[2] 范我[1] 李文新[2] 

机构地区：[1]苏州大学核医学院,苏州215007 [2]中国科学院原子核研究所

出　　处：《苏州医学院学报》2000年第12期1095-1097,共3页Acta Academiae Medicinae Suzhou

基　　金：国家自然科学基金资助课题! (2 96 710 33)

摘　　要：目的　研究俄歇电子发射体67Ga-EDTMP对人骨肉瘤细胞株 (HOS - 86 0 3)的辐射效应 ,探讨67Ga作为原发肿瘤和骨转移癌内照射治疗核素的可能性。方法　用成集落实验和透射电镜研究受照细胞的形态变化。结果　发现67Ga-EDTMP对肿瘤细胞有明显的杀伤和抑制增殖作用 ,并随剂量的加大 ,抑制效率增加 ;倒置显微镜下细胞集落数量减少 ,集落偏小 ,细胞稀疏。电镜下胞浆中空泡形成 ,细胞溶解、坏死 ,细胞核固缩 ,出现典型的细胞凋亡改变 ,形成凋亡小体。Objective To investigate the radiation effect of Auger electron emitter 67 Ga-EDTMP on the human osteosarcoma cell line (HOS-8603). Methods The clonogenic assay and transmission electron microscope were used.Results After internal exposure to 67 Ga, the size of the colonies was diminished along with the increase of the dose,although it was not a sensitive cell line to the external β and γ radiation.The morphological change was observed with transmission electron microscope, compared with the control cells, the irradiated cells have more cytoclasis and cytolysis, the pykonosis and apoptosis corpus were also observed.Conclusion The 67 Ga may be a prospective therapeutic radionuclide used for the tumor treatment and used as palliation of bone pain induced by the metastasis.

关 键 词：俄歇电子发射体 骨肉瘤 Ga67-EDTMP 

分 类 号：R738.1[医药卫生—肿瘤] R817[医药卫生—临床医学]