细胞凋亡与Alzheimer病的关系  

The Relationship between the Apoptosis and Alzheimer Disease

作　　者：夏春林[1] 金涛[1] 魏福堂[1] 

机构地区：[1]苏州大学解剖学教研室暨神经细胞生物学研究室,苏州215007

出　　处：《苏州医学院学报》2000年第12期1104-1106,共3页Acta Academiae Medicinae Suzhou

摘　　要：目的　建立Alzheimer病 (AD)大鼠动物模型 ,探讨细胞凋亡与AD发生发展的关系。方法　大鼠以氯化铝溶液灌胃制备AD动物模型 ,通过Y形迷宫实验 ,判断AD动物模型是否建立成功。TUNEL法原位检测动物模型脑细胞凋亡情况。结果　迷宫实验发现灌铝大鼠短期学习记忆能力和灌铝前及对照组相比明显下降 (P <0 .0 0 1)。TUNEL法检测显示AD动物模型脑细胞凋亡明显增多 ,但海马各区之间未见显著差别。结论　通过氯化铝灌胃可建立较为简便可行的AD动物模型 ;Objective To establish the Alzheimer (AD) disease rat model through pre treatment with aluminium chloride to investigate the possible relationship between the apoptosis and AD.Methods After pre treatment with aluminium chloride, the model of demential was established. The AD animal models were concluded to be successful or not,through the performance of Y-shape maze task. Investigating the apoptosis in situ in brain of AD animal models with the terminal deoxyribonucleotidyl transferase mediated digoxigenin-11-deoxyuridine triphosphate biotin nick-end labeling (TUNEL) method.Results The learning ability of aluminium-treated rats was lower than that of those without aluminium-treated ( P< 0.001) and the controls ( P <0.001). These showed that the AD animal models were successfully established. The apoptotic cells were apparently higher in brains of AD animal models than those in the controls, but there was no difference among the regions of hippocampal formation. Conclusion (1) Aluminium-treated rats are easy-developed and feasible AD animal models. (2) The apoptosis may play an important role in neurodegeneration of AD

关 键 词：ALZHEIMER病 动物模型 细胞凋亡 

分 类 号：R749.160.2[医药卫生—神经病学与精神病学]