ApoE^(-/-);SAP-Tg小鼠模型的构建  

Establishment of the ApoE^(-/-);SAP-Tg mouse model

作　　者：吴腾[1] 邢丽英[1] 勾红菊[2] 兰天[1] 郑凌云 顾取良[3] 温定文[1] 王丽京[1] 

机构地区：[1]广东药学院血管生物研究所,广东广州510006 [2]南方医科大学 基础医学院,广东广州510515 [3]广东药学院基础学院,广东广州510006

出　　处：《广东药学院学报》2013年第5期539-542,共4页Academic Journal of Guangdong College of Pharmacy

基　　金：国家自然科学基金(31271455;81200308;31100852;31200861)

摘　　要：目的为研究SAP蛋白在动脉粥样硬化中的作用及机制,拟构建ApoE-/-;SAP-Tg小鼠模型。方法分别选取6~8周龄的雄性ApoE-/-小鼠与血清淀粉样蛋白P转基因(SAP-Tg)6周龄的雌性小鼠杂交,得到的杂交后代以剪尾方式提取基因组DNA,用PCR法检测小鼠的基因型。结果雄性ApoE-/-小鼠与雌性SAP-Tg小鼠杂交得F1代后自交,在F2代获得ApoE-/-;SAP-Tg基因型小鼠,模型构建成功。结论将ApoE-/-小鼠与SAP-Tg小鼠杂交2代后成功建立了ApoE-/-;SAP-Tg小鼠模型,并可通过筛选出的F2代ApoE-/-小鼠和ApoE-/-;SAP-Tg小鼠交配进行繁育筛选工作。Objective To establish the ApoE^-/-; SAP-Tg mouse model. Methods Female SAP-Tg mice were mated with male ApoE^-/- mice. The progeny of mice were genotyped by PCR. Results After female SAP-Tg mice were mated with male ApoE^-/- mice for three generations, ApoE^-/-; SAP-Tg mice were established. Conclusion ApoE^-/- ;SAP-Tg mice are successfully constructed through ApoE^-/- mice mating with SAP-Tg mice.

关 键 词：ApoE^- - 小鼠 血清淀粉样蛋白P 血清淀粉样蛋白P过表达(SAP—Tg)小鼠 动物模型 动脉粥样硬化 

分 类 号：R-332[医药卫生]