TinII intron, an enhancer to affect the function of the cytoplasmic male sterility related gene T in Brassica juncea  

作　　者：JIN ZhuPing WU LingLing CAO JiaShu CHEN ZhuJun PEI YanXi 

机构地区：[1]School of Life Science,Shanxi University [2]School of Chemical Engineering and Environment,North University of China [3]Institute of Vegetable Science,Zhejiang University

出　　处：《Science China(Life Sciences)》2013年第12期1107-1112,共6页中国科学（生命科学英文版）

基　　金：supported by the National Natural Science Foundation ofChina(31071809)to Pei YanXi

摘　　要：The T gene, which was cloned from the mitochondria of tumorous stem mustard （Brassica juncea var. tumida）, is a cytoplas- mic male sterility （CMS）-related gene that can produce two transcripts, T1170 and T1243. The latter is transcribed with the un- cleaved intron Tinll. In our previous study, transgenic Arabidopsis thaliana plants over-expressing the T1243 transcript （OE-T1243） showed a severe male-sterile phenotype, whereas OE-Tll70 plants did not. However, the functional mechanism of the T gene in B. Juncea remained unknown. In this study, microscopic analyses of paraffin sections of anthers confirmed that OE-T1243 plants did not produce normal pollen, whereas OE-T1170 plants did. We analyzed the transcription of 15 anther development-related genes and found that transcript levels of nozzle/sporocyteless and barely any meristem 1 and 2 were markedly lower in OE-T1243 plants than those in wild type, while the transcript levels of these genes in OE-Tll70 plants were unchanged. To investigate the potential roles of TinH, we inserted the TinH sequence upstream of a minimal region （-60） of the cauliflower mosaic virus 35S promoter fused to the 5＇ end of the 13-glucuronidase （GUS） reporter gene. Analysis of the transgenic plants suggested that TinH acted as an enhancer to significantly increase GUS expression. The potential action mechanism is that the TinH intron acts as an enhancer to affect the function of the CMS-related gene T.The T gene,which was cloned from the mitochondria of tumorous stem mustard(Brassica juncea var.tumida),is a cytoplasmic male sterility(CMS)-related gene that can produce two transcripts,T1170 and T1243.The latter is transcribed with the uncleaved intron TinII.In our previous study,transgenic Arabidopsis thaliana plants over-expressing the T1243 transcript(OE-T1243)showed a severe male-sterile phenotype,whereas OE-T1170 plants did not.However,the functional mechanism of the T gene in B.Juncea remained unknown.In this study,microscopic analyses of paraffin sections of anthers confirmed that OE-T1243 plants did not produce normal pollen,whereas OE-T1170 plants did.We analyzed the transcription of 15 anther development-related genes and found that transcript levels of nozzle/sporocyteless and barely any meristem 1 and 2 were markedly lower in OE-T1243 plants than those in wild type,while the transcript levels of these genes in OE-T1170 plants were unchanged.To investigate the potential roles of TinII,we inserted the TinII sequence upstream of a minimal region(60)of the cauliflower mosaic virus 35S promoter fused to the 5′end of theβ-glucuronidase(GUS)reporter gene.Analysis of the transgenic plants suggested that TinII acted as an enhancer to significantly increase GUS expression.The potential action mechanism is that the TinII intron acts as an enhancer to affect the function of the CMS-related gene T.

关 键 词：cytoplasmic male sterility （CMS） alternative splicing ENHANCER INTRON 

分 类 号：Q943[生物学—植物学]