结直肠癌线粒体DNA拷贝数改变及4977片段缺失  

COPY NUMBER CHANGE AND 4 977bp DELETION OF mtDNA IN COLORECTAL CANCER

作　　者：王志津[1] 郭晓明[2] 张鏊歆 张振华[1] 徐伟[1] 王小鹏[1] 

机构地区：[1]中国人民解放军第三〇五医院消化科,北京100017 [2]中国人民解放军第三〇九医院老年病科,北京100091 [3]中国人民解放军第三〇五医院病理科,北京100017

出　　处：《河北医科大学学报》2013年第11期1372-1375,共4页Journal of Hebei Medical University

摘　　要：目的探讨结直肠癌(colorectal cancer,CRC)线粒体DNA(mitochondrial DNA,mtDNA)拷贝数异常、线粒体DNA 4 977 bp大片段缺失与TNM分期和分化程度的关系。方法选取118例石蜡标本大肠癌组织,分别提取总DNA。以ND1和β-actin为目的基因,进行SYBR Green荧光定量PCR扩增,并用PCR扩增方法检测4 977片段缺失情况,探讨它们与不同TNM分期和分化程度之间的关系。结果 CRCⅠ和Ⅱ期癌组织平均拷贝数(2ND1/β-actin)分别为128.42±31.25和115.12±47.15,Ⅲ和Ⅳ期癌组织平均拷贝数为105.22±16.35和99.45±28.46。Ⅰ和Ⅱ期的拷贝数明显高于Ⅲ和Ⅳ期的拷贝数(P<0.01),癌组织低、中、高分化的平均拷贝数分别为101.34±41.35、112.33±42.32和127.22±31.23(P<0.01),4 977片段缺失率为15.25%(18/118),线粒体DNA4977bp缺失与患者的分期呈负相关,与分化程度无明显关系。结论线粒体DNA 4 977 bp缺失在大肠癌的早期阶段起到了重要作用,随着肿瘤的进展,拷贝数逐渐减少,线粒体DNA拷贝数的变化及大片段缺失在肿瘤的发病机制中可能起一定作用。Objective To study the relationship between mitochondrial DNA copy number abnormalities,4 977bp deletion with TNM and differentiation of colorectal cancer. Methods Total DNA was extracted from cancerous tissues in 118 colorectal carcinoma samples. Using the SYBR Green I quantitative polymerase chain reaction, the mitochondrial to nuclear DNA ratio was determined by quantification of ND1 and β-actin. The detection of 4 977bp deletion was tested by PCR amplification. Results Mean 2ND1/β-actin DNA ratios for cancerous tissues of CRC in early stage （ I and 11 ） were 128.42 ± 31.25 and 115. 12 ±47. 15, respectively, and were 105.22 ±16.35 and 99.45 ± 28.46 respectively in advanced staged（ m and IV ） CRC tissues. The copy number in early stage was higher than that in advomced stage. The mean mtDNA copy number from poorly, moderately and well differentiated samples were respectively 101.34 ± 41.35,112.33 ± 42.32,127.22 ± 31.23 （P 〈 0.01 ）. The ratio of 4 977bp deletion was 15.25% （18/118） , and the deletion of mitochondrial DNA 4 977bp was negatively correlated with the stage, and was no significant relationship with the differentiation degree . Conclusion The mitochondrial DNA 4 977 bp deletion plays an important role in the early stages of colorectal cancer, and mtDNA copy numbers were negatively correlated with the differentiation degree , the variation of copy number and large deletions in mtDNA may play a role in the pathogenesis of tumors.

关 键 词：结直肠肿瘤 DNA 线粒体 聚合酶链反应 

分 类 号：R735.3[医药卫生—肿瘤]