球型芽孢杆菌2362株对两种按蚊幼虫杀伤效果的比较研究  

Comparative study of killing effects of Bacillus sphaericus strain 2362 on two Anopheles mosquitoes

作　　者：于莎莎[1] 王英[1] 艾国平[1] 赵清[1] 吴红渠 

机构地区：[1]第三军医大学军事预防医学院热带医学研究所,重庆400038 [2]湖北省生物农药工程研究中心,湖北武汉430064

出　　处：《中国热带医学》2013年第10期1177-1180,共4页China Tropical Medicine

基　　金：国家自然科学基金面上项目(No.81271875);军队课题(CWS12J017)资助项目

摘　　要：目的了解球形芽孢杆菌2362株(Bs)对大劣按蚊及斯氏按蚊幼虫的毒力作用,为生物杀虫剂的使用提供科学依据。方法利用Probit analysis方法在实验室对球型芽孢杆菌2362株杀伤大劣按蚊和斯氏按蚊幼虫的效果进行了生物测定,并对两种按蚊的敏感性进行了比较研究。结果球形芽孢杆菌2362株对大劣按蚊幼虫48 h和72h的LC50值为(0.0151±0.0029)mg/l和(0.0110±0.0014)mg/l,LC95值为(0.0960±0.0099)mg/l和(0.0529±0.0193)mg/l;对斯氏按蚊Hor株幼虫48h和72h的LC50值为(0.0184±0.0015)mg/l和(0.0162±0.0014)mg/l,LC95值为(0.0850±0.0219)mg/l和(0.0620±0.0114)mg/l。结论球形芽孢杆菌2362株对大劣按蚊和斯氏按蚊均有较好的杀伤效果,大劣按蚊对Bs的敏感性似较斯氏按蚊幼虫略高,且作用持续时间也稍长。Abstract：Objective To investigate the virulence ofBaciUus sphaericus （Bs）strain 2362 against the 4th instar larvae of Anopheles dirus and An. stephensi in laboratory and to provide the scientific basis for mosquito control by using biological insecticides. Methods Bioassay was carried out to compare the toxicity of Bacillus sphaericus strain 2362 against two Anopheles larvae by probit analysis in the laboratory. Results The LCo of An. dirus larvae was （0.0151 ±0.0029）mg/1 at 48 hours and （0.0110 ± 0.0014）mg/1 at 72 hours, whereas the LC was （0.0960 ± 0.0099）mg/1 at 48 hours and （0.0529 ± 0.0193）rag/1 at 72 hours. However,the LCs0 of An. stephensi larvae was （0.0184 ± 0.0015）mg/l at 48 hours and （0.0162 ± 0.0014）mg/l at 72 hours,LC was （0.0850± 0.0219）mg/1 at 48 hours and （0.0620 ± 0.0114）mg/l at ？2 hours,respectively. Conclusions Bacillus sphaericus strains 2362 can kill both of the two Anopheles mosquitoes＇larvae efficiently. An. dirus larvae were more susceptible to Bs than the larvae of An. stephensi. The duration of Bs toxicity to An. dirus larvae was seemly longer than that to An. stephensi.

关 键 词：关键词 球型芽孢杆菌 大劣按蚊 斯氏按蚊 幼虫 生物测定 

分 类 号：R384.1[医药卫生—医学寄生虫学]