HPLC法测定木糖醇及低碳多元醇氢解液的研究  

Determination of xylitol and the lower polyols derived from xylitol hydrogenolysis by HPLC method

作　　者：陈安猛[1] 杨健健[1] 杨晓瑞[1] 梁金花[1] 朱建良[1] 

机构地区：[1]南京工业大学生物与制药工程学院,江苏南京211816

出　　处：《现代化工》2013年第11期131-133,共3页Modern Chemical Industry

基　　金：国家"863"计划重大项目(2011AA02203)

摘　　要：建立了高效液相色谱（HPLC）分析木糖醇及其氢解液的方法,并采用外标法定量分析乙二醇、1,2-丙二醇和1,3-丙二醇.结果表明：最佳分析条件为流动相V（水）∶V（乙腈）=9∶1,pH为5.3,流速为0.600 mL/min,柱温为80℃,进样量为10 μL.在此条件下得到木糖醇质量浓度为0.16 ～ 100.00 g/L,乙二醇为0.78～50.00 g/L,1,2-丙二醇及1,3-丙二醇为0.39～25.00 g/L,各组分的线性关系R为0.999 7～0.999 9,方法的平均回收率为98.31％～101.11％,相对标准偏差（RSD）为0.85％ ～2.35％.HPLC equipped with ShodexSC1011 （8.0 mm ID x 300 mm） column and refractive index detector is developed to determine xylitol and lower polyols derived from xylitol hydrogenolysis and the quantification of ethylene glycol, 1,2-propylene glycol and 1,3-propylene glycol is performed by external standard method. The optimal chromatographic conditions are as follows：the mobile phase water-acetonitrile （9： 1, v/v） is pumped at a flow rate of 0. 600 mL/min, operating temperature of 80℃, the injection colume of 10 μL. In which condition, a linear response （r 〉 0. 999 ） is observed for xylitol ranging from 0. 16 g/L to 100.00 g/L, ethylene glycol ranging from 0.78 - 50. 00 g/L, 1, 3-propanediol and 1,2-propanedio ranging from 0. 39 -25.00 g/L, the method procides of 98.31% - 101.11% with RSD in the range of 0. 85% - 2. 35%.

关 键 词：高效液相色谱 木糖醇 氢解 多元醇 

分 类 号：O657.72[理学—分析化学]