犬场蚊子与犬细小病毒  

Detection of Canine Parvovirus in Kennel Mosquitoes

作　　者：杨德威[1] 宋延华[1] 刘福安[1] 

机构地区：[1]华南农业大学动物医学系,广东广州510642

出　　处：《广东畜牧兽医科技》2000年第4期16-18,共3页Guangdong Journal of Animal and Veterinary Science

摘　　要：2 0 0 0年 4月 ,应用套式PCR技术从犬场蚊子的血液样品中检测出犬细小病毒。将分离到的犬细小病毒VP2 Y34基因片段克隆到PMD1 8 T载体 ,其病毒基因组CPV VP2 Y34序列测定结果显示与作者在GENEBANK发表的肺病变犬细小病毒CPV HN 1株有The presence of a vertically transmitted Canine parvovirus strain CPV/HN-1 in mosquitoes was revealed when screened with a Nested PCR in April 2000.Utilizing 2 stretches of nucleotide sequences repotred for the VP2 gene of canine parvovirus primers,DNA of the samples were subjected to a nested PCR procedure.The amplified Cloned VP2-Y3?Y4 fragment was cloned into a PMD18-T vector and the nucleotide sequence determined.Partial sequencing of the CPV-VP2-Y3?Y4 DNA fragment showed 99% nucleotide homology with that of the canine parvovirus,strain CPV/HN-1.

关 键 词：犬细小病毒 蚊子 套式PCR 检测 

分 类 号：S858.292[农业科学—临床兽医学]