超声波法提取长白楤木叶皂苷及其组分分析  

Ultrasonic Extraction and Composition Identification of Saponins from Aralia continentalis Leaves

作　　者：冯颖[1] 孙士慧[1] 赵琳娜[1] 范文丽[2] 

机构地区：[1]沈阳农业大学食品学院,辽宁沈阳110866 [2]沈阳农业大学园艺学院,辽宁沈阳110866

出　　处：《食品科学》2013年第22期5-9,共5页Food Science

基　　金：国家自然科学基金青年科学基金项目(31000803)

摘　　要：在单因素试验的基础上,采用L9(34)正交试验设计对长白楤木叶皂苷的提取工艺进行优化。结果表明,最佳提取工艺参数为体积分数70%的乙醇为提取剂、提取剂用量50mL、超声波提取时间30min、超声波功率200W,提取率为26.78mg/g。富集提取后的皂苷,以HPD-100树脂纯化、硅胶柱层析、制备液相进行分离,获得一个皂苷单体,经核磁共振法鉴定为楤木叶皂苷A。One-factor-at-a-time method and L9（34） orthogonal array design were employed to optimize the extraction pro- cess of saponins from ,4ralia contenentalis leaves. The results showed that the optimal conditions of ultrasonic extraction were determined as follows： ethanol concentration of 70%, material-to-liquid ratio of 1：50 （g/mL）, extraction duration of 30 rain and ultrasonic power of 200 W. Under the optimal extraction process, the extraction yield of saponins was up to 26.78 mg/g. Mac- roporous adsorption resin HPD-100, silica gel, SephadexLH-20 and preparative HPLC were used to separate the extracted saponin and congmuyenoside A was identified by NMR.

关 键 词：长白楤木 皂苷 超声波 提取 鉴定 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]