羊胎盘免疫活性小分子肽的分离纯化  

Separation and Purification of Small-Molecule Pe ptides with Immunomodulating Activity from Goat Placenta

作　　者：刘隆兴[1] 任兴宏[1] 汤禾静 唐照勇[1] 何成明[2] 方廖琼[1] 

机构地区：[1]西南大学生物技术学院,重庆400715 [2]重庆理工大学药学与生物工程学院,重庆400054

出　　处：《食品科学》2013年第22期273-276,共4页Food Science

摘　　要：目的:从羊胎盘中分离纯化出具有免疫活性的小分子肽。方法:采用常规方法制备羊胎盘超滤液,利用反相高效液相色谱仪(RP-HPLC)对羊胎盘提取液进行分离纯化及纯度分析,并利用E-玫瑰花环实验对各组分进行免疫活性评价,利用Edman降解法对其组分进行N端测序。结果:利用RP-HPLC工艺成功分离出纯度较高的3种小分子肽,并确定其中两者具有免疫活性;对活性较强的肽进行测序、检索,结果表明为未知组分。结论:RP-HPLC是分离、分析活性肽类较理想的手段之一,并证实了羊胎盘含有具有免疫活性的小分子肽。The purpose of this study was to isolate and purify small-molecule peptides with immunomodulating activ- ity from goat placenta. Ultrafiltration fraction from goat placenta was prepared by a conventional method, followed by isolation, purification and analysis by reversed phase-high performance liquid chromatography （RT-HPLC）. The im- munomodulating activity of its components was measured by E-rosette test and the N-terminal sequence was measured by Edman degradation reaction. Three small-molecule peptides with high purity were obtained, two of which were im- mune-active. The results of sequencing and retrieving showed that they were new peptides. RT-HPLC was one of the optimal methods to purify and analyze bioactive peptides. The small-molecule peptides from goat placenta revealed strong, immune activity.

关 键 词：羊胎盘小分子肽 反相高效液相色谱 E-玫瑰花环 

分 类 号：TQ460.7[化学工程—制药化工] R931.74[医药卫生—生药学]