环氧合酶-2基因敲低对足细胞相关蛋白表达的影响  

The effect of COX-2 gene knockdown on podocyte associated proteins

作　　者：邓振兴[1] 孔维信[1] 张林[1] 孙怀鑫[1] 丁露露[1] 郝传明[2] 陈靖[3] 

机构地区：[1]上海交通大学医学院苏州九龙医院肾内科 [2]复旦大学附属华山医院肾内科 [3]江苏大学临床医学院,镇江212013

出　　处：《陕西医学杂志》2013年第11期1446-1449,共4页Shaanxi Medical Journal

基　　金：江苏省苏州市科技计划项目(SS0716)

摘　　要：目的:观察足细胞环氧合酶-2(COX-2)基因敲低对足细胞相关蛋白表达的影响。方法:以条件永生性小鼠足细胞株为研究对象,分为足细胞正常对照组、足细胞COX-2基因敲低组和足细胞阴性转染组,用流式细胞仪检测足细胞凋亡水平,用Western bolt检测COX-2、Nephrin、Podocin、CD2相关蛋白(CD2AP)的表达水平。结果:与足细胞正常对照组及阴性转染组相比,足细胞COX-2基因敲低组COX-2蛋白表达明显降低、细胞凋亡率明显增高,而Nephrin、Podocin、CD2AP蛋白表达则显著降低。结论:足细胞COX-2基因敲低可以导致足细胞凋亡,且足细胞Nephrin、Podocin、CD2AP蛋白的表达下调。Objective：To investigate the effect of cyclooxygenase-2 （COX-2） gene knockdown on podocyte associated proteins .Methods：The conditionally immortalized murine podocytes were divided into three groups ：nor-mal control group ,podocytes treated with siRNA and podocytes treated with negative siRNA .The level of podocyte apoptosis was measured by flow cytometry .The proteins expression of COX-2、Nephrin、Podocin、CD2AP were detec-ted by western blotting .Results ：The expression of COX-2 protein was decreased and the podocytes apoptosis were increased in podocytes treated with siRNA .And the protein expressions of Nephrin、Podocin、CD2AP in siRNA group were significantly lower than those in normal control group and negative siRNA group .Conclusion ：The COX-2 gene knockdown induces podocytes apoptosis .The protein expression of Nephrin、Podocin、CD2AP in podocyte treated with siRNA are downregulated .

关 键 词：足细胞 细胞凋亡 免疫学 @基因敲低 环氧合酶2 免疫学 

分 类 号：R363[医药卫生—病理学]