硝酸镓对胃癌细胞增殖与凋亡的作用  

Effect of gallium nitrate on proliferation and apoptosis of gastric cancer cells

作　　者：王学哲[1,2] 于洪儒[1] 王洪新[1] 张蕴莉[2] 

机构地区：[1]辽宁医学院,辽宁锦州121001 [2]辽宁医学院附属第一医院,辽宁锦州121001

出　　处：《解放军医学院学报》2013年第11期1185-1188,共4页Academic Journal of Chinese PLA Medical School

摘　　要：目的通过硝酸镓、顺铂单药及二者联合用药对胃癌BGC823细胞作用,观察其对胃癌BGC823细胞诱导凋亡、抑制增殖的影响。方法通过对胃腺癌BGC823细胞培养及干预,采用流式细胞仪技术对凋亡细胞进行测定;MTT技术对肿瘤细胞增殖进行测定。结果 MTT及流式细胞仪检测结果显示:硝酸镓对BGC823细胞作用呈剂量-时间依赖效应;小剂量硝酸镓与顺铂配伍应用能大大提高顺铂作用效应,配伍高浓度组在24 h、48 h、72 h的抑制率为70.0%、71.4%、72.3%。结论硝酸镓对胃癌细胞的作用与顺铂无明显差异,为肿瘤治疗及制备抗肿瘤药物提供新的途径。Objective To assess the inhibitory effect of gallium nitrate, cisplatin and their combination on proliferation and apoptosis of gastric cancer （BGC823） cells. Methods The BGC823 cells were cultured and treated with gallium nitrate, cisplatin or their combination. Apoptosis and proliferation of BGC823 cells were detected by flow cytometry and MTT assay, respectively. Results MTT assay and flow cytmetry showed that gallium nitrate inhibited the proliferation of BGC823 cells in a time- and dose- dependent manner. Small dose of combined gallium nitrate and cisplatin could greatly increase the inhibitory effect of cisplatin on the proliferation and apoptosis of BGC823 cells. The inhibition rate of combined gallium nitrate and cisplatin was 70.0%, 71.4% and 72.3% at 24 h, 48 h and 72 h, respectively. Conclusion The effect of gallium nitrate and cisplatin on proliferation and apoptosis of BGC823 cells is not significantly different and can thus provide a new approach to the drug treatment of gastric cancer and to the preparation of anti-tumor drugs.

关 键 词：硝酸镓 胃癌细胞 细胞增殖与凋亡 

分 类 号：R735.2[医药卫生—肿瘤]