抗红花花粉蛋白单克隆抗体的研制  

Research and Preparation of Anti-safflower Pollen Protein Monoclonal Antibodies

作　　者：张庆生[1] 李波[1] 张凤兰[1] 裴新荣[1] 陈志蓉[1] 王钢力[1] 达晶[1] 王春香 林瑞超[1] 

机构地区：[1]中国食品药品检定研究院,北京100050 [2]北京康为世纪生物科技有限公司,北京102206

出　　处：《中国医院用药评价与分析》2013年第10期911-913,共3页Evaluation and Analysis of Drug-use in Hospitals of China

基　　金：国家科技部十二五"重大新药创制"科技重大专项子课题"红花注射液大品种技术改造"课题中的子课题"过敏物质基础及其不良反应机理研究"(编号:2011ZX09201-201);国家自然科学基金"Ⅰ型超敏反应中Egr-2对肥大细胞信号转导的调控机制研究"(编号:81072446)

摘　　要：目的:研究红花中过敏原性物质的检测和评价方法,为筛查红花制剂过敏原残留、优化红花制剂工艺、预防红花制剂过敏反应的发生提供新的研究思路和方法。方法:以水煮醇沉法提取红花花粉蛋白,用花粉蛋白免疫小鼠,筛选特异性细胞克隆,扩增、纯化制备抗红花蛋白单克隆抗体。采用酶联免疫吸附剂测定(ELISA)方法检测单克隆抗体对红花抗原的特异性识别情况。结果:3株单克隆抗体均能特异性识别红花抗原。结论:抗红花蛋白单克隆抗体对研究红花过敏反应的发生机理、优化红花制剂工艺及降低过敏反应发生具有重要意义。OBJECTIVE：To investigate approaches for the detection and assessment of allergens in safflower productions so as to shed light on the screening of allergen residues in safflower productions, optimizing of safflower preparation process and prevention of safflower - induced hypersensitivity. METHODS： The safflower pollen proteins were extracted by water-boiling-and-alcohol-precipitation method, i. e, the mice were immunized with safflower pollen protein, and then the specific cell clones were screened, amplified and purified to prepare anti-safflower pollen protein monoclonal antibodies. ELISA method was applied to identify specificity recognition of monoclonal antibodies on safflower pollen protein. RESULTS： Three strains of monoclonal antibodies could all specifically recognize safflower pollen antigen. CONCLUSION ： The anti-safflower pollen protein monoclonal antibodies are of great significance in the research of mechanism of safflower-induced hypersensitivity, optimization of preparation process of safflower and reduction of safflower-induced hypersensitivilty.

关 键 词：红花 过敏原 单克隆抗体 

分 类 号：R965[医药卫生—药理学]