清热解毒中药有效组分配伍对内毒素致大鼠感染性脑水肿的保护作用  

Protective Effect of Heat-Clearing Antitoxicant Herb Components Compatibility on Infectious Brain Edema in Rats induced by Lipopolysaccharide

作　　者：姚明江[1] 刘建勋[1] 张鹏[1] 杨斌[1] 徐立[1] 王光蕊[1] 

机构地区：[1]中国中医科学院西苑医院基础医学研究所,中药药理北京市重点实验室,北京100091

出　　处：《世界中医药》2013年第10期1129-1131,1136,共4页World Chinese Medicine

基　　金：国家自然科学基金项目(编号:30772755);"重大新药创制"科技重大专项(编号:2009ZX09103-317);科技部十二五重大新药创制课题(编号:2012ZX09301002-004)

摘　　要：目的:探讨清热解毒中药黄芩、栀子等有效组分配伍而成的注射液(TLQN注射液)对内毒素(LPS)所致大鼠感染性脑水肿的脑保护作用及其可能机制。方法:70只SD大鼠随机分为正常组、模型组、清开灵组(3 mL/kg)、地塞米松组(10 mg/kg)、TLQN注射液低、中、高剂量组(24、47.5、95 mg/kg)。经颈内动脉注射LPS(1 mg/kg)建立感染性脑损伤模型,各给药组在注入LPS后立即尾静脉注射相应药物。造模前以及造模6h后各测量一次体温。取各组脑组织标本,干湿法测定脑组织含水量,甲酰胺法检测脑组织伊文思蓝(EB)水平,HE染色后观察各组脑组织病理形态变化,酶联免疫法(ELISA)检测各组血清TNF-α、IL-6含量。结果:模型组体温、脑组织含水量和EB含量明显高于正常组(P<0.01),皮质及海马区存在广泛的神经元、胶质细胞损伤,血清TNF-α及IL-6水平显著升高(P<0.01);TLQN注射液可明显降低模型大鼠体温(P<0.05),减少脑组织含水量及EB含量(P<0.05,0.01),改善脑组织神经元、胶质细胞损伤,显著降低血清TNF-α和IL-6水平(P<0.05)。结论:清热解毒中药有效组分配伍对LPS致大鼠感染性脑损伤有保护作用,其机制可能与抑制TNF-α和IL-6等细胞因子过度产生有关。Objective： To explore the protective effect of Heat- Clearing antitoxicant herb（ Scutellaria baicalensis Georgi,Gardenia jasminoides Ellis,et al） component compatibility（ TLQN Injection） on infectious brain edema in rats induced by lipopolysaccharide（ LPS） and its possible therapeutic mechanism. Methods： A total of 70 SD rats were divided randomly into model group,control group,Qingkailing group（ 3 mL / kg）,Dexamethasone group（ 10 mg / kg）,TLQN low-dose（ 24 mg / kg）,middle-dose（ 47. 5 mg / kg） and highdose（ 95 mg / kg） group. Models of acute infectious brain edema of rats were prepared by injecting LPS（ 1 mg / kg） via left internal carotid artery,and the drugs was injected at the same time as LPS was given via tail vein. 6h after the models were established,the body temperature,the water content and EB content in the brain was measured,the pathological changes was observed and the serum TNF-α、IL-6 was mesured by ELISA. Results： The body temperature,the water content and EB content in the brain were higher in model group than in control group,as well as injured cortical and hippocampacal neurons and glial cells,the serum TNF-α and IL-6 levels in model group were higher than control group; TLQN injection can significantly decrease the body temperature,reduce water content and EB content,improve injured neurons and glial cells,and decrease the serum TNF-α and IL-6 levels. Conclusion： The Heat-Clearing antitoxicant herb component compatibility injection may lighten the degree of infectious brain injury induced by LPS,the possible mechanism could be it can inhibit the overproduction of cytokines such as TNF-α and IL-6.

关 键 词：清热解毒 组分配伍 脂多糖 感染性脑水肿 炎症 

分 类 号：R742.7[医药卫生—神经病学与精神病学]