海产品中副溶血弧菌三种快速检测方法的比较研究  

Comparative study of three rapid methods for Vibrio parahaemolyticus detection in aquatic products

作　　者：裴琳 卢京光 栾成章 

机构地区：[1]山东省青岛市药品检验所,山东青岛266071

出　　处：《中国卫生检验杂志》2013年第9期2092-2093,2104,共3页Chinese Journal of Health Laboratory Technology

基　　金：国家863计划(2008AA100805)

摘　　要：目的比较三种方法快速测定海产品中副溶血弧菌的专属性和灵敏度。方法选取荧光定量PCR、LAMP、显色培养基法分别对阳性对照菌和阴性对照菌共13株进行特异性、灵敏度试验,并对结果进行比较分析。结果 PCR、LAMP法检测副溶血弧菌与显色培养基法相比具有更好的专属性;LAMP法的灵敏度最高,菌液浓度为10 cfu/ml时即可检出;PCR法次之,为800 cfu/ml;显色培养基法最低,为6500 cfu/ml。结论三种副溶血弧菌快速检测方法中,PCR、LAMP法具有更好的专属性;LAMP法的灵敏度最高,PCR法次之,显色培养基法最低。Objective To compare the specificity and sensitivity of three rapid methods for Vibrio parahaemolyticus detection in aquatic products.Methods The specificity and sensitivity were detected seperately by Fluorescence quantitative PCR,LAMP and color medium method on 13 positive and negative reference strains,and comparative analysis was performed to the results.Results PCR LAMP methods show high specificity compared to color medium method in V.parahaemolyticus detection.LAMP can dectect 10 cfu / ml of V.parahaemolyticus,showing the highest sensitivity,and the sensitivity of PCR is 800 cfu / ml which takes the second place.The color medium method has the lowerst sensitivity which is 6500 cfu / ml.Conclusion Among three rapid detection methods for V.parahaemolyticus detection in aquatic products,PCR LAMP methods show high specificity.LAMP shows the highest sensitivity,PCR takes the second place,and the color medium method is the lowerst.

关 键 词：副溶血弧菌 荧光定量PCR 环介导等温扩增(LAMP) 快速检测 

分 类 号：TS207.4[轻工技术与工程—食品科学]