乳腺癌外周血淋巴细胞亚群检测及其预后意义  

Detection and clinical significance of lymphocyte subsets in peripheral blood of breast cancer

作　　者：朱赤红[1] 许沈华[1] 凌雨田[1] 

机构地区：[1]浙江省肿瘤医院,浙江杭州310022

出　　处：《中国卫生检验杂志》2013年第14期2957-2960,共4页Chinese Journal of Health Laboratory Technology

摘　　要：目的检测乳腺癌患者以及正常女性外周血淋巴细胞亚群表达水平,探讨乳癌患者与正常女性的差异,分析乳癌患者外周血淋巴细胞亚群表达水平与患者临床病理以及预后的关系。方法采用流式细胞术检测124例乳腺癌患者和79例正常女性外周血中CD3+、CD4+、CD8+、CD19+和NK细胞所占比例,结合临床病理和随访资料进行统计分析。结果乳腺癌患者的CD3+和CD8+细胞表达水平比正常女性低(P<0.01),CD4+和CD19+细胞含量比正常女性高(P<0.01)。临床病理因素分析表明CD3+细胞表达水平低、NK细胞表达水平高的患者与有肿瘤神经侵犯成正相关(P<0.01),NK细胞表达水平较高者与有脉管瘤栓成正相关(P<0.05)。CD8+细胞表达水平高的患者无进展生存时间较长(P<0.05)。结论低表达CD8+细胞的乳腺癌患者预后不佳(P=0.008)。Objective To detect peripheral blood lymphocyte subsets expression level, discuss the differences between breast cancer patients and normal controls, as well as the association between the proportion of peripheral blood lymphocyte subsets of breast cancer patients and clinical pathology and prognosis. Methods Flow cytometry was applied to measure the proportion of lymphocyte subsets（ CD3 ＋ , CD4 ＋ , CD8 ＋ , CD19 ＋ and NK cells） in peripheral blood of 124 breast cancer patients and 79 healthy controls. The experiment resuh was analyzed in combination with clinical pathology data and follow -up data. Results The percentage of CD3 ＋ and CD8 ＋ cells in breast cancer patients was lower than that in controls （P 〈0.01 ）, while that of CD4 ＋ and CD19 ＋ cell was higher when compared with controls （ P 〈 0.01 ）. Breast cancer patients with a higher CD3 ＋ cell proportion and a lower NK cell proportion had a lower risk of perineural invasion. And patients with a higher NK cell proportion had a higher risk of blood vessel invasion（P 〈 0.05 ）. Breast cancer patients with a higher level of CD8 ＋ cells had a longer disease - free survival time （P 〈 0.05）. Conclusion Low - level CD8 ＋ cells suggest the poor prognosis of breast cancer patients （P = 0.008）.

关 键 词：乳腺肿瘤 淋巴细胞亚群 CD8+ 

分 类 号：R737.9[医药卫生—肿瘤]