下调miR-21表达抑制鼻咽癌CNE2细胞增殖和侵袭  

miR-21 downregulation attenuates cell proliferation, migration and invasion in nasopharyngeal carcinoma

作　　者：周克兵[1] 谷刚[1] 曹昕[1] 

机构地区：[1]南华大学附属南华医院急诊科,湖南衡阳421002

出　　处：《中国癌症杂志》2013年第11期863-867,共5页China Oncology

基　　金：国家自然科学基金(No:81101526)

摘　　要：背景与目的:miR-21在人类多种肿瘤中异常高表达。本研究探讨干扰miR-21表达对鼻咽癌CNE2细胞增殖、迁移和侵袭的影响。方法:使用脂质体将miR-21 inhibitor转染CNE2细胞,以无关序列(NC inhibitor)作为阴性对照。采用qRT-PCR技术验证miR-21 inhibitor转染的CNE2细胞中miR-21的表达水平;通过MTS法、细胞划痕、Transwell侵袭实验观察下调miR-21表达对CNE2细胞增殖、迁移和侵袭的影响。结果:转染miR-21 inhibitor的CNE2细胞中miR-21的表达明显下调,并且呈浓度依赖性。表明转染miR-21 inhibitor能有效抑制CNE2细胞中miR-21的表达。转染miR-21 inhibitor的CNE2细胞与对照细胞相比,增殖速度明显减慢,差异有统计学意义(P<0.05)。细胞划痕实验显示,下调miR-21表达抑制CNE2细胞迁移(P<0.05)。Transwell侵袭实验结果显示,下调miR-21表达抑制CNE2细胞侵袭(P<0.05)。结论:miR-21能促进鼻咽癌细胞增殖、迁移和侵袭,其可能在鼻咽癌的发生、发展中发挥重要作用。Background and purpose： miR-21 is ovexpressed in various types of human cancers. This study was designed to investigate the effect of miR-21 knockdown on cell proliferation, migration and invasion of nasopharyngeal carcinoma （NPC） cell line CNE2. Methods： CNE2 was transfected with miR-21 inhibitor by LipofectamineTM2000. Meanwhile CNE2 was transfected with NC inhibitor as negative control, qRT-PCR was used to detect the miR-21 expression in these cells. The effects of miR-21 downregulation on cell proliferation, migration and invasion were evaluated by MTS, wound-healing Transwe11 and invasion assays. Results： miR-21 expression was remarkably downregulated in miR-21 inhibitor-transfected cells in concentration-dependent manner, indicating transfection with miR-21 inhibitor can effectively reduce expression level of miR-21 in CNE2 cells. Transfection of miR-21 inhibitor into CNE2 cells led to a significant decrease in cell proliferation rate compared with control cells （P〈0.05）. miR-21 downregulation results in reduction of cell migration（P〈0.05）. Moreover, the cell invasion by Transwell invasion assay was reduced in miR-21-downregulated cells relative to control cells. Conclusion： miR-21 can promote cell proliferation, migration and invasion of NPC cells. And it maybe plays an important role in tumorigenesis and development of NPC.

关 键 词：鼻咽癌 MIR-21 细胞增殖 细胞侵袭 

分 类 号：R739.63[医药卫生—肿瘤]