IgG在人脑胶质瘤中的表达及其与OPN、MMP-2、MMP-9、P16的关系  

The relationship of IgG expression with OPN, MMP-2, MMP-9 and P16 in human glioma

作　　者：薛雨健 张杰[2] 汪明玉[3] 张时超[4] 张贺[1] 牛娜[4] 

机构地区：[1]潍坊医学院外科学教研室,山东潍坊261053 [2]潍坊医学院解剖学教研室,山东潍坊261053 [3]潍坊医学院内科学教研室,山东潍坊261053 [4]潍坊医学院病理学教研室,山东潍坊261053

出　　处：《解剖科学进展》2013年第6期513-515,519,共4页Progress of Anatomical Sciences

基　　金：国家自然科学基金(No.81100885);山东省优秀中青年科学家科研奖励基金(No.BS2011SW051)

摘　　要：目的检测IgG在人脑胶质瘤中的表达及其与OPN、MMP-2、MMP-9、P16表达的关系。方法免疫组化检测IgG、OPN、MMP-2、MMP-9、P16在80例胶质瘤组织中的表达,原位杂交和RT—PCR检测IgG mRNA在组织和胶质瘤细胞系U373细胞中的表达。结果胶质瘤组织和U373细胞系细胞中有IgG蛋白及IgG mRNA表达,IgG、OPN、MMP-2、MMP-9和P16的表达阳性率在不同级别胶质瘤组均有显著性差异,IgG与OPN、MMP-2、MMP-9的表达呈正相关,与P16表达呈负相关。结论胶质瘤中IgG、OPN、MMP-2、MMP-9高表达,P16低表达,可能在胶质瘤的发生发展中起一定作用。Objective To investigate the expression of IgG and its relationship with OPN, MMP-2, MMP-9 and P16 in htunan glioma. Methods The expressions of IgG, OPN, MMP-2, MMP-9 and PI6 were detected by immunohistochemistry. IgG mRNA was detected with in situ hybridization in tissue, and with RT-PCR in U373 cells. Results IgG protein and IgG mRNA were expressed positively in glioma and U373 cells. There were significant differences for the positive rates of IgG, OPN, MMP-2, MMP-9 in glioma tissues between different grades. There was a positive correlation between IgG expression and those of OPN, MMP-2 and MMP-9, with negative correlation between IgG expression and that of P16. Conclusion Overexpressed IgG, OPN, MMP-2 and MMP-9, and downexpressed PI6 in glioma indicating that they may be involved in pathogenesis of glioma.

关 键 词：胶质瘤 IGG OPN MMP-2 MMP-9 P16 

分 类 号：R730.264[医药卫生—肿瘤]