RNA干扰沉默IGF-IR基因治疗卵巢癌的实验研究  

Experimental study of insulin-like growth factor-I receptor silencing mediated by RNA interference in ovarian cancer therapy

作　　者：安媛[1] 孙壮状[1] 卢美松[1] 

机构地区：[1]哈尔滨医科大学附属第一医院生殖医学科,黑龙江哈尔滨150001

出　　处：《中国优生与遗传杂志》2013年第12期35-37,5,共4页Chinese Journal of Birth Health & Heredity

基　　金：黑龙江省教育厅科学技术研究项目资助;编号12531344

摘　　要：目的研究RNA干扰技术沉默IGF-IR在卵巢癌靶向治疗中的意义。方法以IGF-IR为目的基因,设计合成siRNA重组质粒;利用Real-time PCR和Western blot方法,分别从mRNA水平及蛋白水平检测IGF-IR的表达;通过MTT实验检测细胞增殖情况。结果本实验成功构建了三个表达载体pSih IGF-IR-1、pSih IGF-IR-2和pSih IGF-IR-3,转染卵巢癌OVCAR3细胞,48h后,IGF-IR的mRNA及蛋白水平均明显降低,并且显著抑制了OVCAR3细胞的体外增殖。结论应用RNA干扰技术,能够高效、特异的沉默IGF-IR基因的表达,抑制肿瘤生长。针对卵巢癌IGF-IR的RNAi技术,可能为卵巢癌的临床治疗开拓一条新的途径。Objective ： To investigate the possibility of IGF - IR small interfering RNA （siRNA） as a promising anticancer agent. Methods： The technology of RNA interference was used to silence IGF - IR gene expression in the human ovarian cancer cell line OVCAR3. Real - time PCR and western blot assay were used to observe the mRNA and protein expression of IGF - IR, and then evaluate its effects on proliferation of tumor cells in vitro. Results ： Three siRNA sequences for the IGF - IR gene were cloned into expres- sion plasmids and transfected into OVCAR3 ceils. The mRNA and protein expression of IGF - IR were significantly decreased at 48h after transfection, leading to the potent suppression of tumor cell proliferation in vitro. Conclusions： IGF - IR - specific siRNA suppresses tumor growth, which could define a potential therapeutic target.

关 键 词：卵巢癌 胰岛素样生长因子I型受体 RNA干扰 

分 类 号：R737.31[医药卫生—肿瘤]