硝酸钠对发状念珠蓝细菌生长的促进作用  

Effect of NaNO_3 on the growth and photosynthesis of Nostoc flagelliforme cells

作　　者：吕和鑫[1] 贾士儒[1] 肖玉朋[1] 袁南南[1] 戴玉杰[1] 

机构地区：[1]天津科技大学天津市工业微生物重点实验室,天津300457

出　　处：《中国酿造》2013年第10期13-16,共4页China Brewing

基　　金：长江学者和创新团队发展计划资助(IRT1166)

摘　　要：研究了添加不同浓度的藻类培养常用无机氮源NaNO3对发状念珠蓝细菌生长、光合速率、胞外多糖积累、蛋白质含量、色素含量以及磷酸根与硝酸根消耗的影响。结果表明,添加NaNO3对发状念珠蓝细菌的生长具有促进作用,2.0g/L的NaNO3对细胞生长的促进作用最大,藻细胞干重达到0.83g/L,净光合速率最大,随着添加浓度的继续增加,细胞干重达到平衡。细胞呼吸作用在NaNO3为2.5g/L时最大。细胞蛋白质含量随NaNO3浓度的增大逐渐升高,与此相应,细胞对NO3-与PO43-的利用率也逐渐增高。在不同NaNO3浓度下,培养15d后的培养液的pH值差异不显著,但是添加组的最终pH值明显比不加硝酸钠的空白对照要小。In this study, the effect of NaNO3 on the growth, photosynthetic rate and exopolysaccharides production of Nostoc. tlagelliforme were investigated in the flasks. The results showed that increased NaNO3 could significantly promote the growth of N. flagelliforme. The results indicated that 2g/L NaNOa was the optimal concentration for biomass and exopolysaccharides productions, while the net photosynthetic rate reached maximal and the biomass reached 0.83g/L. When the concentration of NaNO3 increased, the biomass reached a plateau, whereas the respiration rate got a maximal at the 2.5g/L NaNO3. The protein contents of N. Bagelliforme cells were increased gradually along with the increase of NaNO3 concentration.

关 键 词：发状念珠蓝细菌 硝酸钠 氮源 生物量 光合速率 

分 类 号：Q939.1[生物学—微生物学]