靶向沉默caveolin-1基因对胃癌耐药细胞增殖和迁移影响  

Silencing of the caveolin-1 gene inhibits proliferation and migration of multidrug resistant gastric adenocarcinoma cells

作　　者：张晔[1] 曲秀娟[1] 刘云鹏[1] 徐玲[1] 赵明芳[1] 侯科佐[1] 滕月娥[1] 

机构地区：[1]中国医科大学附属第一医院肿瘤内科,辽宁省沈阳市110001

出　　处：《世界华人消化杂志》2013年第32期3532-3536,共5页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;Nos.81270036;30901736;81201802;辽宁省自然科学基金资助项目;No.2013021057~~

摘　　要：目的:探讨靶向沉默caveolin-1(cav-1)基因对阿霉素(adriamycin,ADR)诱导的人胃癌耐药细胞SGC7901/ADR增殖和迁移作用的影响及可能机制.方法:通过小干扰R N A转染胃癌细胞S G C7901/A D R,以未转染细胞为正常对照组,转染siRNA Control为阴性对照组,RT-PCR法检测靶向沉默cav-1基因的效果,MTT法检测各组SGC7901/ADR细胞的增殖能力,Transwell小室迁移实验检测各组SGC7901/ADR细胞的迁移能力,Western blot检测细胞周期相关蛋白Cyclin D1、Cyclin A1和Cyclin E蛋白表达水平.结果:靶向沉默cav-1基因显著抑制胃癌SGC7901/ADR细胞增殖,P<0.05;靶向沉默cav-1基因显著下调细胞周期相关蛋白Cyclin D1和Cyclin A1,但对Cyclin E无影响;同时,靶向沉默cav-1基因显著抑制胃癌SGC7901/ADR细胞迁移能力.结论:靶向沉默cav-1基因可抑制胃癌耐药细胞的增殖和迁移,可能与其抑制细胞周期相关蛋白Cyclin D1、Cyclin A1和迁移相关分子有关.AIM： To investigate the effect of silencing of the caveolin-1 （cav-1） gene on the proliferation and migration of multidrug resistant gastric adeno- carcinoma cells （SGC7901/ADR） and to explore the underlying mechanisms. METHODS： A siRNA specific for the cav-1 gene was transfected into SGC7901/ADR cells us- ing Lipofectamine 2000. The proliferation andmigration of SGC7901/ADR cells were detected by MTT assay and transwell assay, respectively. The mRNA expression of car-1 was determined by RT-PCR. The protein expression of Cyclin D1, Cyclin A1 and Cyclin E was determined by Western blot. RESULTS： Compared with the control group, the mRNA expression of cav-1 was signifi- cantly decreased in SGC7901/ADR cells after transfection （P 〈 0.05）. The proliferation and migration of SGC7901/ADR cells were inhibit- ed significantly after cav-1 silencing. Addition- ally, the protein expression of Cyclin D1 and Cyclin A1 was obviously inhibited. However, silencing of cav-1 did not affect Cyclin E ex- pression. CONCLUSION： Silencing of the cav-1 gene in- hibits the proliferation and migration of human gastric cancer SGC7901/ADR cells possibly by decreasing the expression of Cyclin D1 and Cy- clin A1.

关 键 词：CAVEOLIN-1基因 RNA干扰 胃癌 肿瘤增殖 肿瘤迁移 

分 类 号：R735.2[医药卫生—肿瘤]