温石棉及其代用纤维对鼠细胞EGFR及Survivin表达的影响  

Effect of chrysotile and its substitute on EGFR and Survivin expression of hamster cells

作　　者：邓丽娟[1] 王洪州[1] 王利民[1] 董发勤[2] 

机构地区：[1]四川绵阳四〇四医院川北医学院附属第二医院呼吸内科,四川绵阳621000 [2]西南科技大学校长办公室,四川绵阳621000

出　　处：《中国医药导报》2013年第33期4-6,10,共4页China Medical Herald

基　　金：国家自然科学基金(编号49502025;40072020)

摘　　要：目的探讨温石棉及其代用纤维对鼠细胞表皮生长因子受体(EGFR)及凋亡抑制基因(Survivin)表达的影响。方法将温石棉及其代用品暴露于中国仓鼠肺细胞(Chinese hamster lung cell,V79),根据不同实验粉体(A^F组以及阴性对照组)以及不同粉体浓度(1~10 mg/L)分为37组,使用免疫组织化学法检测EGFR及Survivin的表达,并使用SPSS 21.0对肿瘤基因EGFR及Survivin的前后表达进行对比分析。结果 A组和B组的仓鼠肺细胞中肿瘤基因EGFR及Survivin的表达增强较为显著,与其他组相比,差异有统计学意义(P<0.05);Survivin以及EGFR表达随着粉体浓度的增加,其OD值也呈上升趋势。结论温石棉及其代用纤维对于鼠细胞肿瘤基因EGFR及Survivin表达有显著的增强作用。Objective To discuss the effect of chrysotile and its substitute on EGFR and Survivin expression in hamster cells.Methods Chinese hamster lung cells （V79 cells） were divided into 37 groups by different powder （A-F groups and control group） and its concerntrantion （1-10 mg/L）.The EGFR and Survivin expression in Chinese hamster lung cells were tested by immunohistochemistry method when they were exposed to Chrysotile and its substitute.And then EGFR and Survivin statistics were compared by SPSS 21.0.Results The oncogene expression of EGFR and Survivin in group A and B were upregulated significantly in Chinese hamster lung cells,compared with other groups,the differences were statistically significant （P ＜ 0.05）.As the concentration of powder increased,OD value of EGFR and Survivin increased.Conclusion Chrysotile and its substitute can significantly influence the EGFR and Survivin expression in hamster cells.

关 键 词：温石棉 代用品 肿瘤基因 表皮生长因子受体 凋亡抑制基因 

分 类 号：R734.2[医药卫生—肿瘤]