天花粉蛋白诱导胃癌细胞MKN-45凋亡的研究  

Induction of apoptosis by trichosanthin in gastric cancer cell MKN-45

作　　者：涂水平[1] 江石湖[1] 乔敏敏[1] 王立夫[1] 张曙[1] 袁耀宗[1] 吴云林[1] 吴裕忻[1] 

机构地区：[1]上海第二医科大学瑞金医院消化科,上海200025

出　　处：《癌症》2000年第12期1105-1108,共4页Chinese Journal of Cancer

基　　金：卫生部消化疾病重点实验室青年基金!(No: 9806010)资助

摘　　要：目的 :研究天花粉蛋白（ trichosanthin,TCS）对胃癌细胞的作用，探讨其治疗胃癌的可能作用机制。方法 :采用生长曲线、集落形成实验、 MTT法、电镜、 TUNEL染色和流式细胞仪等技术，研究 TCS对胃癌细胞 MKN－ 45的增殖抑制和诱导凋亡作用。结果： TCS能够明显抑制胃癌细胞 MKN－ 45的生长和集落形成， 1μ g/ml和 0.1μ g/ml TCS作用 MKN－ 45后，细胞集落形成率分别为 (6.63± 1.31)％和 (14.68± 1.27)％，明显低于对照组的 (23.67± 2.76)％Objective: The current study was designed to explore the mechanism of actions of trichosanthin(TCS) on gastric cancer cell MKN 45. Methods: Cell proliferation ability, colony formating rate of gastric cancer cell MKN 45 treated by TCS were examined by using growth curve and colony formating assay. The cytotoxicity of TCS on MKN 45 cells was determined by using MTT assay. Apoptosis and cell cycle changes of MKN 45 induced by TCS were investigated by using TUNEL method and flow cytometry. Results: TCS signifcantly inhibited the growth of MKN 45. The rates of inhitition were correlated with the concentration of TCS. Colony formating rates of MKN 45 treated with 1μ g/ml TCS and 0.1μ g/ml TCS were (6.63± 1.31)％ and (14.68± 1.27)％ respectively, which were much lower than that of control group (23.67± 2.76)％ (P

关 键 词：天花粉蛋白 细胞凋亡 胃癌细胞 MKN-45 

分 类 号：R735.2[医药卫生—肿瘤]