细胞毒化疗对急性白血病原位凋亡诱导作用的体内研究  

Cytotoxic chemotherapy induced in situ apoptosis in patients with acute leukemia

作　　者：李晓[1] 金朝晖[1] 浦权[1] 杨梅如[1] 刘薏芝[1] 陶英[1] 石军[1] 蒋秦燕[1] 

机构地区：[1]上海市第六人民医院血液科,上海200233

出　　处：《癌症》2000年第12期1143-1145,共3页Chinese Journal of Cancer

摘　　要：目的：观察细胞毒化疗对急性白血病细胞原位凋亡的影响。方法：应用 DNA原位末端标记（ ISEL）方法，检测初发及经治的 31例急性白血病骨髓塑包切片内细胞原位凋亡数量及特征，以 10例缺铁性贫血为对照，并进行自身组间对照。结果：初治 AL凋亡减少，与对照组相比有显著差异（Objective: The current study was designed to observe the impact of cytotoxic chemotherapy on in situ apoptosis in acute leukemia(AL). Methods: Plastic embedded BM sections of 31 cases of untreated or treated AL were detected by DNA in situ end labelling (ISEL )technique with 10 cases of iron defficiency anemia as control. Results: Apoptosis was much lower in untreated AL group than that in control group, and positive correlation was showed between the number of apoptotic cell and the blast decrease percentage after effective chemotherapy. Conclusion: Apoptosis escape was existed in untreated AL and one of the possible mechanism of cytotoxic chemotherapy is to promote leukemic cell apoptosis. Thus apoptosis detection could be used as an index to assess the prognosis of acute leukemia.

关 键 词：急性白血病 ISEL 细胞毒化疗 原位凋亡 

分 类 号：R733.71[医药卫生—肿瘤]