变性与非变性电泳对牛羊乳蛋白质差异比较研究  

The study of the differences between cow and goat milk proteins by SDS-PAGE and native-PAGE

作　　者：宋宏新[1] 刘静[1] 张歌[1] 李红心[1] 

机构地区：[1]陕西科技大学生命科学与工程学院,陕西西安710021

出　　处：《陕西科技大学学报（自然科学版）》2013年第6期109-113,共5页Journal of Shaanxi University of Science & Technology

基　　金：陕西省教育厅科研计划项目(2013JC03)

摘　　要：主要采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳法(SDS-PAGE)和非变性聚丙烯酰胺凝胶电泳法(Native-PAGE)对新鲜牛羊乳及其酪蛋白清蛋白的区别进行分析检测,对比研究两种电泳方法测定结果的差异,并用两种方法分别检测了掺入不同浓度牛乳的羊乳样品.结果显示SDS-PAGE法对牛羊乳酪白质的分离效果好,该条件下牛羊乳的区别主要是酪蛋白αs2-CN和αs1-CN,而native-PAGE法则是对清蛋白分离效果较好,该条件下的主要区别是牛乳较羊乳多两条β-乳球蛋白.两种电泳方法对掺入不同浓度的羊乳样品的检测阈值均为5%,但native-PAGE效果更明显.This experiment analysis and detection fresh cow and goat milk and casein protein, albumin protein by sodium dodecyl sulphate polyacrylamide gel electrophoresis （SDS-PAGE） and native polyacrylamide gel electrophoresis （native-PAGE）, comparative the results differ- ences of two electrophoresis methods. And were detected the goat milk samples doped with different concentrations of cow milk by both methods. The results showed that by SDS- PAGE casein protein are separated better, and the main differences are as2-CN and asl-CN, while native-PAGE separate albumin protein better, and the main difference is that cow milk has two β-lactoglobulin,but goat milk hasn＇t. And the detection threshold of those two kinds of electrophoresis methods of detected the goat milk samples doped with different concentra- tions of cow milk is 5 %. But the native-PAGE is more effective.

关 键 词：SDS—PAGE Native—PAGE 乳蛋白质 羊乳牛乳蛋白质差别 

分 类 号：TS252.4[轻工技术与工程—农产品加工及贮藏工程]