海红果酒中甲醇比色测定条件优化  

Optimization of measurement conditions of methanol in hai-hong wine with colorimetric method

作　　者：杨辉[1] 黄丽梅[1] 罗建华 

机构地区：[1]陕西科技大学生命科学与工程学院,陕西西安710021 [2]怀来县贵族庄园葡萄酒业有限公司,河北怀来075400

出　　处：《陕西科技大学学报（自然科学版）》2013年第6期114-117,123,共5页Journal of Shaanxi University of Science & Technology

基　　金：陕西省科技厅重大科技创新项目(2011ZKC11-2);科技部科技成果转化项目(2013GB2G000473);陕西科技大学博士科研启动基金项目(BJ09-14)

摘　　要：针对比色法测定海红果酒中甲醇标准曲线线性差的问题,通过单因素实验,对影响甲醇测定的4个主要因素进行了研究,最佳的测定条件为:草酸-硫酸溶液用量为0.6mL,无甲醇的乙醇溶液浓度为40%,静置时间为13min,显色温度为40℃,在此条件下甲醇标准曲线的R2值为0.997.In order to get a good linear standard curve for determination of methanol in hai- hong wine with the colorimetric method, the single factor test was used to optimize the measurement conditions. Results show that there are four factors significantly affecting the determination of methanol. The optimal measurement conditions are as follows： the amount of the solution of oxalic acid-sulfuric acid is 0.6 mL; the ethanol concentration 400/00 in meth- anol-free solution, reacting for 13 minutes after the solution of KMnO4-H3PO4 mixed with the solution of methanol sample, coloration temperature of 40 ℃. Under these conditions, the standard curve of methanol is a good straight line with correlation coefficient of 0. 997.

关 键 词：甲醇 分光光度法 灵敏度 

分 类 号：TS261.7[轻工技术与工程—发酵工程]