狂犬病减毒脂质体口服冻干活疫苗的制备及理化性质的检测  

The Preparation of Liposome Freeze- dried Oral Live Vaccines of Rabies Attenuated Strain and Its Physical and Chemical Property

作　　者：翟少华[1] 骆建敏[2] 赵森[1] 马素贞[1] 高攀[1] 苏晓慧[1] 夏婷婷[1] 张美玲[1] 李建飞[1] 简子健[1] 

机构地区：[1]新疆农业大学动物医学学院,乌鲁木齐830052 [2]新疆大学理化测试中心,乌鲁木齐830046

出　　处：《新疆农业科学》2013年第11期2130-2135,共6页Xinjiang Agricultural Sciences

基　　金：新疆维吾尔自治区高技术研究发展计划项目(200611107);新疆维吾尔自治区普通高校重点学科(基础兽医学)科研启动基金

摘　　要：【目的】分析研发的狂犬病减毒脂质体口服冻干活疫苗的脂质体形态、粒径分布状态、疫苗抗原包封率,确定狂犬病减毒脂质体口服冻干活疫苗的制备工艺。【方法】(1)以大豆卵磷脂、胆固醇、十八胺、VE等物质为原料,采用薄膜分散法制备空白脂质体,采用电子显微镜和激光粒径分布仪测定空白脂质体的形态与粒径分布;(2)采用冷冻干燥方法将狂犬病rSRV9减毒株与空白脂质体等体积混合后冻干,制备狂犬病减毒脂质体口服冻干活疫苗;(3)采用BCA蛋白定量法检测狂犬病减毒脂质体口服冻干活疫苗的抗原包封率。【结果】制备空白脂质体形态为大单室脂质体;平均粒径为378 nm;疫苗包封率为71.19%。【结论】薄膜分散-冻干方法制备狂犬病减毒脂质体口服冻干活疫苗,抗原包封率高、性状稳定。[ Objective ] The liposome freeze -dried oral live vaccine of rabies attenuated strain was pre- pared and its morphology of liposome, distribution and encapsulation efficiency of vaccine antigen was evalua- ted for determining the preparation process of this kind of vaccine in this article. [ Method] （1） The soy leci- thin, cholesterol, octadecylamine, and vitamin E was used as raw materials, and the blank liposome was pre- pared by film dispersion, and the morphology and particle size distribution of blank liposome was evaluated by electronic microscope and laser detector MASTER2000; （2） The rabies rSRV9 strains and the blank liposome were mixed together in the equal - volume by freeze - drying method to prepare liposome freeze - dried oral live vaccine of rabies attenuated strain ; （3） The antigen encapsulation efficiency of rabies attenuated oral livevaccine was detected by BCA protein quantitative method. [ Results] The prepared blank liposome was large single chamber, and the average size of particles was about 378 nm, and the encapsulation efficiency was about 71.19%. [ Conclusion] The liposome freeze -drying oral live vaccine of rabies attenuated strain was pre- pared by film dispersion and freeze - drying method was stable and had high efficiency of antigen encapsula- tion.

关 键 词：狂犬病rSRV9减毒株 脂质体 口服 活疫苗 粒径分布 包封率 

分 类 号：S854.43[农业科学—临床兽医学]