3种猪孕酮受体基因(pgr)多态性分析  

Polymorphism of pgr gene in three pig populations

作　　者：高利华[1] 马国辅[1] 陈超[1] 邢军[1] 张建生[1] 吴井生[1] 

机构地区：[1]江苏农林职业技术学院,江苏句容212400

出　　处：《湖南农业大学学报（自然科学版）》2013年第6期621-625,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：江苏镇江市科技项目(NY2009016);江苏农林职业技术学院项目(2012[8])

摘　　要：采用混合基因组DNA池PCR扩增和测序技术,对小梅山猪、枫泾猪、大白猪pgr基因的外显子区域进行扫描,共发现1个单核甘酸多态性位点(SNP)。外显子1序列中1 319 bp处发生C→T的碱基突变,即C1319T,未引起氨基酸的变化,属于同义突变;针对突变位点,建立错配PCR–限制性长度多态性(RFLP)方法进行检测,发现3种猪的pgr基因可以分为AA、BB、AB 3个基因型,小梅山和枫泾猪有AA、AB 2种基因型,大白猪中有BB、AB 2种基因型。All exons in progesterone receptor（pgr） gene in Xiaomeishan, Fengjing and Large White pig populations were screened using mixed genome DNA pool and direct sequencing methods. The results showed that only one site exhibits nucleotide polymorphism （SNP）, which is in exonl of pigpgr gene at 1 319 bp with base mutation from C to T （C1319T） This mutation do not cause the change of amino acid residue, which is a synonymous mutation. Based on this mutation, mismatched PCR-RFLP method was established to analyze polymorphism of pgr gene in the above mentioned pig populations. According to PCR-RFLP, three pig populations is divided into AA, AB and BB genotypic types, in which AB types exist in Xiaomeishan and Fengjing pig populations, and BB, AB types in Large White pig population.

关 键 词：猪孕酮受体基因慨力 小梅山猪 枫泾猪 大白猪 多态性 

分 类 号：S828.8[农业科学—畜牧学]