茎瘤芥黑斑病菌rDNA ITS区序列分析  

Sequence Analysis of ITS Region of rDNA of Brassica juncea Coss. var. tumida Tsen et Lee Black Spot Disease

作　　者：刘红芳[1] 陈发波[1] 杨永强[1] 

机构地区：[1]长江师范学院生命科学与技术学院,重庆408100

出　　处：《湖北农业科学》2013年第20期5044-5046,共3页Hubei Agricultural Sciences

基　　金：重庆市教育委员会科学技术研究项目(KJ111307)

摘　　要：以茎瘤芥(Brassica juncea Coss.var.tumida Tsen et Lee)黑斑病病株为试材,对黑斑病菌5.8 S rDNA及其侧翼ITS区序列进行克隆、测序和比对分析。结果表明,5个供试病菌碱基序列同芸薹链格孢的碱基序列相似度达到99.68%,不存在大于3 bp的碱基差异;而与甘蓝链格孢和萝卜链格孢的碱基差异较明显,均存在大于3 bp的碱基差异,且存在大量的缺失片段。初步确定引起茎瘤芥黑斑病的病原菌为芸薹链格孢。Taking Brassica juncea Coss. var. tumida Tsen et Lee black spot disease as materials, 5.8 S rDNA and its flanking ITS were cloned, sequenced and aligned. The results showed that the sequence of 5 pathogen were almost identical with that of Alternaria brassicae. Similarity degree reached 99.68%. There was no differences more than 3 bp base. There were obvious differences between the sequence of A. brassicicola and A. japonica, more than 3 bp different base and a lot of deletions. It was prelimiarily dertermined that the pathogen caused the black spot disease on Brassica juncea Coss. var. tumida Tsen et Lee was Alternaria brassicae.

关 键 词：茎瘤芥(Brassica JUNCEA Coss var tumida Tsen et Lee) 黑斑病 ITS 芸薹链格孢 

分 类 号：S436.3[农业科学—农业昆虫与害虫防治]