培养方法及胎牛血清浓度对人胎儿成纤维细胞体外生长的影响  

The Effect of Culture Method and Concentration of Fetal Bovine Serum on the Growth of Human Fetal Fibroblasts Cultured in Vitro

作　　者：程腾[1] 李佳佳[1] 贺小英[1] 刘娟[1] 马利兵[1] 

机构地区：[1]内蒙古科技大学数理与生物工程学院,内蒙古包头014010

出　　处：《信阳师范学院学报（自然科学版）》2013年第4期523-526,共4页Journal of Xinyang Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(31160245);内蒙古自治区高等学校青年科技英才支持计划资助项目

摘　　要：采用单细胞培养法和组织块培养法从原代培养人胎儿成纤维细胞,并在不同体积分数胎牛血清(FBS)(0%、4%、8%、10%、12%)的条件下观察人胎儿成纤维细胞培养的状况并采用台盼蓝染色法进行活细胞计数.结果表明,早期由组织块培养法原代培养获取的细胞形态要比单细胞培养法原代培养获取的细胞稍好些,且在细胞传代后极少出现无法贴壁的死细胞.FBS对hFFs生长影响比较明显.在实际应用中,在体积分数8%FBS下,采用组织块培养法进行人胎儿成纤维细胞体外培养,效果良好.The effect of single cell culture method and tissue block culture method on the growth of human fetal fi- broblasts cultured in vitro was compared, and the best effect of which concentration of FBS on the growth of human fetal fibroblast cultured in vitro was studied. Human fetal fibroblasts were primarily cultured by single cell cuhure method and tissue block culture method, and the condition of human fetal fibroblasts on bovine serum （FBS） （0% , 4%, 8%, 10% , 12% ） was observed. Then by means of the method of trypan blue staining, the viable cells were counted. The results showed that, in the primary passage, the morphology of cell by tissue block culture method is better than that of cell cultured by single cell method, and dead cells of unable to stick wall were seldom appeared after passage. FBS had significant influence on the growth of hFFs. In the real application, when the concentration of FBS was 8%, using tis- sue block culture method to cultivate human fetal fibroblasts had a good effect.

关 键 词：人胎儿成纤维细胞 单细胞培养法 组织块培养法 胎牛血清 

分 类 号：S828[农业科学—畜牧学]