长链非编码RNA MEG3等在肺癌组织中的表达  

Expression of long non-coding RNA MEG3 ST8SIA3 and TUG1 in lung cancer

作　　者：李苹[1] 杨凯云[2] 曾龙剑 徐俪烜[1] 姚树祥[1] 吴锡南[1] 何越峰[1] 

机构地区：[1]昆明医科大学公共卫生学院,云南昆明650500 [2]昆明医科大学第三附属医院胸心外科

出　　处：《环境与健康杂志》2013年第11期988-990,共3页Journal of Environment and Health

基　　金：云南省自然科学基金(2009CD081);云南省科技厅-昆明医科大学应用基础研究联合专项资金(2010CD181)

摘　　要：目的探讨长链非编码RNA MEG3、ST8SIA3和TUG1在肺癌中的表达。方法选择47例肺癌患者的癌组织和癌旁正常组织,利用TRIzol提取总RNA,SYBR Green实时荧光定量PCR检测MEG3、ST8SIA3和TUG1 RNA的相对表达量。结果 MEG3和TUG1在癌组织中经对数转换后的相对表达量分别为4.74±2.31和12.90±1.36,均低于癌旁正常组织(分别为6.89±2.19和13.68±1.39),差异有统计学意义(P<0.05);ST8SIA3在癌组织中经对数转换后的相对表达量为13.12±1.55,与癌旁正常组织(13.61±1.72)的差异无统计学意义。Spearman等级相关显示,肺癌组织中ST8SIA3、TUG1的相对表达量与TOPOⅡ呈负相关(分别为r=-0.47,P=0.00;r=-0.42,P=0.01),ST8SIA3的相对表达量与TNM分期呈正相关(r=0.29,P=0.04);未发现其他指标间呈相关性。结论 MEG3、TUG1在癌组织中的相对表达量均降低,提示可能在肺癌的发生发展中起到抑癌因子的作用。Objective To explore the expression of long non-coding RNA MEG3, ST8SIA3 and TUG1 in lung cancer. Methods A total of 47 patients with lung cancer were selected and the cancer tissues and normal tissues were collected, total RNA was extracted with TRIzol, SYBR Green real-time fluoreseenee quantitative PCR was used to detect MEG3, ST8SIA3 and TUG1 RNA relative expression levels. Results MEG3 and TUG1 in cancer tissues relative expression levels were 4.74±2.31 and 12.90±1.36 respectively,lower than the adjacent normal tissue （P〈0.05）; No significant change was seen in ST8SIA3 relative expression. Between ST8SIA3, TUG1 and Topo I[ a negative eorrelation was presented （r=-0.47, P=-0.00; r=-0.42, P=- 0.01）; ST8SIA3 and TNM presented a positive eorrelation （r=0.29, P=0.04）. Conclusion MEG3, TUG1 down-regulated expression in cancer tissues may act as an inhibitory factor in the development of lung eaneer.

关 键 词：肺癌 MEG3 ST8SIA3 TUG1 表达 

分 类 号：R73[医药卫生—肿瘤]