增韧基团-姜黄素单脂增强对前列腺癌的抑瘤作用  

Enhanced inhibitory effect of prostate cancer research with toughening groups-curcumin monoester

作　　者：李海峰[1] 陈方敏[1] 石家齐[1] 李登宝[1] 严波[2] 任德帅[1] 张鹏[1] 陈煜[3] 陈程[1] 

机构地区：[1]贵阳医学院附属医院泌尿外科,550004 [2]贵州省人民医院泌尿外科 [3]贵阳医学院附属医院图书馆

出　　处：《中华实验外科杂志》2013年第12期2493-2495,I0001,共4页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30860284）;贵阳市科学技术攻关计划资助项目（2009筑科农合同字第3-009号）

摘　　要：目的 探讨增韧基团-姜黄素单脂（叔丁氧羰基-苯丙氨酸酯姜黄素单酯）较姜黄素更增强对人前列腺癌的体内抑瘤作用的可行性.方法 建立人前列腺癌PC-3可移植性动物模型.裸鼠共40只,选取肿瘤体积接近的36只随机分成3组.A组（注射生理盐水）;B组（注射姜黄素）;C组（注射叔丁氧羰基-苯丙氨酸酯姜黄素单酯）.隔日1次,共15次.用药后24 h处死裸鼠,剥瘤体,称重并计算抑瘤率.对肿瘤组织行苏木素-伊红（HE）染色和原位末端转移酶标记（TUNEL）检测.结果 成功建立人前列腺癌可移植性动物模型.A、B、C组抑瘤率分别为0％、21.76％、55.18％,各组间差异有统计学意义（P＜0.05）.TUNEL检测结果显示,A、B、C组凋亡指数分别为（4.2±1.3）％、（26.6±7.8）％、（49.2±8.6）％,各组间差异有统计学意义（P＜0.05）.结论 叔丁氧羰基-苯丙氨酸酯姜黄素单酯与姜黄素比较,对人前列腺癌移植瘤有更显著的抑制作用.Objective Discussion of the toughening group-curcumin monoester （tert-butoxycarbonyl-phenylalanine ester curcumin monoester） representing curcumin more enhanced the feasibility about inhibition of human prostate cancer in vivo.Methods After establishment of animal portability models of human prostate cancer of PC-3 cell line with 40 nude mice,which were selected based on tumor volume close to 36 and randomly divided into three groups including A group with injection of normal saline）,group B with injection of curcumin and group C with injection of tert-buto-xycarbonyl group-the phenylalanine ester curcumin monoesters with every other day,a total of 15 times.Four weeks later,nudemice were treated to sacrificing.stripping tumor were weighed and inhibitory rate were calculated.HE staining of tumor tissue and TdT-mediated dUTP nick end labeling （TUNEL） assay were detected.Results The animal models of human prostate cancer portability tumor was successfully established.A,B,C group inhibition rates were 0％,21.76％ and 55.18％ respectively.The difference between the groups were statistically significant （P ＜ 0.05）.In TUNEL assay,A,B and C apoptotic index were （4.2 ± 1.3） ％,（26.6 ± 7.8） ％ and （49.2-± 8.6） ％,differences among the groups were statistically significant （P ＜ 0.05）.Conclusion Inhibiting effect on human prostate cancer xenograft in vivo with tert-butoxycarbonyl pheny lalan-ine ester curcumin monoester on more significantly higher than with pure curcumin.

关 键 词：前列腺癌 叔丁氧羰基-苯丙氨酸酯姜黄素单酯 模型 动物 

分 类 号：R737.25[医药卫生—肿瘤]