华蟾素对大鼠膀胱癌的抑制作用  

Anti-tumor effect of cinobufagin on bladder cancer in SD rats

作　　者：黄后宝[1] 沈群山[1] 王啸[1] 夏东东[1] 邱腾[1] 

机构地区：[1]皖南医学院第一附属医院泌尿外科,安徽芜湖241001

出　　处：《中华实验外科杂志》2013年第12期2514-2516,I0001,共4页Chinese Journal of Experimental Surgery

基　　金：芜湖市科技局农社领域课题项目（2008712）

摘　　要：目的 检测SD大鼠膀胱癌组织的环氧合酶-2（COX-2）、毛细血管密度（MVD）、癌细胞凋亡水平,探讨其与病理分级的相关性.方法 采用N-甲基-N-亚硝基脲（MNU）构建模型,分为空白组、MNU组、华蟾素（Cino）组、阿霉素（ADM）组和卡介苗（BCG）组,每组10只.灌注结束1周后取其膀胱进行苏木素-伊红（HE）染色并检测相关指标.结果 免疫组织化学结果表明,低级别组中,各组COX-2的表达均无差异;Cino组在MVD表达和细胞凋亡两方面与ADM组相似,但均高于BCG组.在高级别组中,Cino组的COX-2和MVD表达均低于其余各组;而细胞凋亡则相反.结论 华蟾素能有效拮抗SD大鼠膀胱癌,且对高级别表达的膀胱癌具有抑制作用.Objective To detect the expression of cyclooxygenase-2 （COX-2）,microvascular density （MVD）,and apoptosis of bladder cancer cells in the SD rats and explore their relationship with pathologic grade.Methods Fifty SD rats were divided randomly into 5 groups,including blank group,N-methyl-N-nitrosourea （MNU） group,cinobufagin （Cino） group,adriamycin （ADM） group and bacillus calmette-guerin （BCG） group.After perfusion for one week,bladder tissues were taken out for hematoxylin and eosin （HE） staining and detection of related indicators.Results Immunohistochemical results showed that COX-2 expression,in lower-grade groups,had no significant difference among different groups.MVD expression and apoptosis of cancer cells in Cino group and ADM group showed no significant difference,but higher than in BCG group.In the higher-grade group,COX-2 and MVD expression in Cino group （2.500 ± 0.535 and 11.750 ± 1.581 respectively） was lower than the rest groups,while apoptosis rate （18.500 ± 0.535） was higher than other groups.Conclusion Cinobufagin can effectively antagonize bladder cancer and suppress the growth of bladder cancer with high grade in SD rats.

关 键 词：华蟾素 膀胱癌 环氧化酶-2 微血管密度 脱噬作用 

分 类 号：R737.14[医药卫生—肿瘤]