严重联合免疫缺陷小鼠肾包膜下异种移植人前列腺增生组织模型的建立  

Establishment of an experimental model of human benign prostatic hyperplasia in severe combined immunodeficiency mice via subrenal capsule xenograft of patient-derived tissues

作　　者：吴建辉[1] 杨阔[2] 杨宇明[3] 马洪顺[4] 徐勇[2] 

机构地区：[1]天津医科大学第二医院天津市泌尿外科研究所天津市第一中心医院泌尿外科,300211 [2]天津医科大学第二医院泌尿外科天津市泌尿外科研究所 [3]天津医科大学第二医院病理科 [4]天津市第一中心医院泌尿外科

出　　处：《中华实验外科杂志》2013年第12期2547-2550,I0002,F0003,共6页Chinese Journal of Experimental Surgery

基　　金：天津市科技计划资助项目（11ZCGYSY02300、12ZCDZSY16300）

摘　　要：目的 利用严重联合免疫缺陷（SCID）小鼠肾包膜下移植法建立人前列腺增生（BPH）组织异种移植模型.方法 将20只4～6周龄SCID小鼠随机分为4组,每组5只.取前列腺术后组织标本,分别移植到小鼠肾包膜下和皮下.实验组为肾包膜下移植,对照组为皮下移植.8～12周后,观察前列腺组织生长,经石蜡包埋后进行常规染色和免疫组织化学鉴定.结果 人BPH组织在SCID小鼠肾包膜下成活率为90％ （18/20）,皮下组仅为40％ （8/20）.异种移植的前列腺组织为实体状、不规则、白色,隆出肾脏表面并嵌入肾实质,伴有血管生成.镜下表现,肾包膜下移植前列腺组织生长旺盛,皮下组前列腺组织腺体萎缩,移行上皮化生.免疫组织化学染色证实为人源性前列腺组织.结论 小鼠肾包膜下移植可高效率建立人BPH组织异种移植动物模型.Objective To establish the xenograft model of human benign prostatic hyperplasia （BPH） by grafting patient-derived tissues beneath the renal capsule of intact male severe combined immunodeficiency （SCID） mice.Methods Twenty SCID mice were randomly divided into 4 groups （n =5each）.Four patient-derived BPH specimens were collected for frozen section analysis.Each sample tissue was cut into multiple 3 mm × 3 mm × 1 mm pieces to be xenografted.The fragments were simultaneously xenografted into the sub-renal capsule （experimental group） and subcutaneous tissue （control group） of one mouse.At week 8-12 after initial implantation,grafts were harvested and fixed to paraffin.For histopathology,routine H＆E and immunohistochemistry staining was carried out.Results The survival rate of BPH xenogtrafts in experimental group was 90％ （18/20）,and that in control group was only 40％（8/20）.The sub-renal capsule xenografts were long out of surface and embedded into the renal parenchyma with angiogenesis.There was no significant increase in hyperplasia at sub-renal capsule xenografts.As compared with control group,atropic changes and transitional metaplasia were found in experimental group.Human BPH tissues were verified in experimental group by using the immunohistochemistry staining.Conclusion The human BPH xenograft model in SCID mice is successfully established.

关 键 词：前列腺增生 异种移植 睾酮 模型 动物 

分 类 号：R-332[医药卫生]