上调视网膜母细胞瘤蛋白结合锌指结构基因1后食管癌细胞基因表达谱的变化  

Change of the expression profile in esophageal carcinoma cells following up-regulated retinoblastoma protein-interacting zinc finger gene 1 gene

作　　者：李栋[1] 张鹏[1] 董尚文[1] 王元国[1] 陈渊[1] 徐聪[1] 

机构地区：[1]天津医科大学总医院心胸外科,300052

出　　处：《中华实验外科杂志》2013年第12期2622-2624,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81201945）;天津市自然科学基金资助项目（10JCYBJC11300）;天津医科大学科学基金青年基金资助项目（2011KY08）

摘　　要：目的 观察转染视网膜母细胞瘤蛋白结合锌指结构基因1（RIZ1）后,食管鳞状细胞癌TE13细胞株基因表达谱的改变.方法 培养TE13细胞株,1∶10传代后培养至对数生长期,脂质体法分别转染pcDNA3.1（＋）和pcDNA3.1（＋）/RIZ1真核表达载体,转染成功后,应用Agilent人类全基因表达谱基因芯片筛选转染前后的差异基因（差异倍数≥2.0或≤0.5）,用聚合酶链反应（PCR）验证结果和对差异基因功能分析.结果 差异基因总共有2123个,其中上调960个,下调1163个,功能主要集中在细胞发育、病毒复制的监管等.RIZ1基因可能参与包括细胞因子受体互相作用等多种信号通路.结论 RIZ1基因通过多种途径发挥作用,为进一步研究RIZ1基因与食管癌间的关系提供了依据.Objective To observe the effects of retinoblastoma protein-interacting zinc finger gene 1 （RIZ1） gene on the expression profile of esophageal squamous cell carcinoma cell line TE13.Methods The TE13 cells were cultured and divided into 10 portions,subcultured and transfected respectively by pcDNA3.1 （＋） and pcDNA3.1 （＋）/RIZ1 using the lipofectamin transfection reagent.After successful transfection,Agilent whole human genome oligo microarray （4 ＊ 44K） was used to screen the effects of RIZ1 on the expression profile of esophageal carcinoma cell line TE13 （foldchange ≥2 or ≤0.5）.The test results was confirmed by real-time quantitative polymerase chain reaction （real-time PCR）.Results The DNA microarray data suggested that RIZ1 transfection induced widespread change of gene expression profile of TE13 cells.The up-regulated and down-regulated genes were amount to 960 and 1 163 respectively.The altered genes belonged to many functional categories,mainly in cell development,regulation of viral reproduction.RIZ1 gene may be involved in some pathways,such as cytokine receptor interaction,TGF-beta signaling pathway.Conclusion Widespread change of gene expression profile occurred after transfection with RIZ1 gene.We postulate that RIZ1 inhibits the tumor via the effect of these genes and involvement in pathways.The study provides the evidence for elucidating the relation between RIZ1 gene and the development and progress of esophageal cancer.

关 键 词：食管鳞状细胞癌 视网膜母细胞瘤蛋白结合锌指结构基因1 基因芯片 

分 类 号：R735.1[医药卫生—肿瘤]