灵长类动物桡神经缺损修复模型的功能学评价  

Functional evaluation on the model of radial nerve defect in primate

作　　者：黄喜军[1] 朱庆棠[1] 江丽[2] 郑灿镔[1] 朱昭炜[1] 胡军[3] 何波[1] 路庆森[1] 许银峰[3] 

机构地区：[1]中山大学附属第一医院显微创伤骨科,广州510080 [2]中山大学附属第一医院骨科研究所,广州510080 [3]中山大学附属第一医院黄埔院区上肢骨科,广州510080

出　　处：《中华实验外科杂志》2013年第12期2674-2676,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30700847、31070869）;广东省自然科学基金资助项目（9251008901000017）;广东省科技计划资助项目（2010B031100006）

摘　　要：目的 探讨灵长类动物桡神经缺损修复后功能学的评价方法.方法 解剖3只猕猴桡神经,建立5只猕猴双侧桡神经25 mm缺损的模型,分别用自体神经（A组）及去细胞猪神经（B组）桥接神经缺损（5侧/组）.术后观察猕猴一般情况及伸腕活动;于术前、术后1周、术后5个月,诱导、测量并计算术后累积最大伸腕角度（DEmax）和伸腕角度恢复率（R）以评价神经功能的修复效果.结果 桡神经在桡侧腕长伸肌第一肌支发出点以近可显露长度为52 ～ 62 mm,直径为3.3～4.1 mm;术后猕猴即时出现垂腕、垂指畸形,一般情况好,术后5个月A、B组DEmax和R的均值分别为120°、96°和99％、69％.结论 该模型安全、简便,可动态、定量评价神经功能恢复情况.Objective To observe the anatomical characteristics of the radial nerve and explore the functional evaluation methods after repairing radial nerve defect in primates.Methods Three adult rhesus monkeys were selected for dissecting radial nerve from axilla to the elbow,and their position and branches were recorded.Five healthy adult rhesus monkeys were selected to establish the model of bilateral radial nerve 25 mm long defect,and nerve defects were bridged with autologous nerve （group A） and acellular porcine nerve （group B） respectively.Wrist extension and general conditions were observed after operation.Postoperative cumulative maximum wrist angle （DEmax） and wrist angle recovery rate （R） were measured and calculated to evaluate the effect of restoration of neurological function by a special fixed chair before operation,1 week and 5 months after operation respectively.Results The first muscular branch of extensor carpi radialis longus （ECRL） in rhesus monkeys was issued 9-14 mm proximal to the lateral condyle of the humerus.The radial nerve could be exposed proximal to the issued point with the length of 52-62 mm,diameter of 3.3-4.1 mm,which could create 25 mm radial nerve defect.Wristdrop and fingerdrop were observed for rhesus monkeys after surgery,with good general conditions and wound healing.After 5 months,the mean of DEmax in group A and group B was 120° and 96° respectively,and R was 99％ and 69％ respectively.Conclusion The radial nerve defect model in the lower arm with safe operation,dynamic observation and quantitative description of the recovery of neurological function,can be used as the standard model of peripheral nerve repair in primate.

关 键 词：周围神经损伤 神经再生 猕猴 模型 动物 

分 类 号：R-332[医药卫生]