脂联素在皮下缝合促进脂肪组织切口愈合中的抗凋亡作用及机制  

Effects and mechanism of adiponectin in promoting adipose tissue wound healing of subcutaneous suture by inhibiting adipocyte apoptosis

作　　者：葛子豪 肖亮[2] 周汉新[1] 占小波 林环[1] 田耕[1] 

机构地区：[1]深圳市第二人民医院肝胆外科,518035 [2]深圳市第二人民医院肿瘤生物治疗科,518035

出　　处：《中华实验外科杂志》2013年第12期2682-2684,共3页Chinese Journal of Experimental Surgery

基　　金：中国博士后科学基金资助项目（2013M531895）;深圳市第二人民医院育苗计划;深圳市科技计划项目（医药卫生类,201202056）;深圳市知识创新计划资助项目（JCYJ20130401114114947、JCYJ20130401114225775）

摘　　要：目的 观察脂联素在皮下缝合的脂肪组织中的表达水平与脂肪细胞凋亡的关系,探讨脂联素在皮下缝合促进脂肪组织切口愈合中的作用及机制.方法 建立猪脂肪组织切口愈合模型,应用逆转录-聚合酶链反应（RT-PCR）方法检测局部脂肪组织脂联素表达水平,应用荧光标记法观察切口局部脂肪细胞凋亡的变化.培养3T3-L1细胞株,应用膜联蛋白V-异硫氰酸荧光素（Annexin V-FITC）凋亡检测法测定不同浓度的脂联素对细胞凋亡的影响.结果 皮下缝合的局部脂肪组织脂联素水平均显著高于对照组（P＜0.05）;术后第3天及第5天皮下缝合组脂肪细胞凋亡数为（29±13）和（15±8）个,分别低于对照组细胞凋亡数[（69±25）、（23±16）个],差异有统计学意义（P＜0.05）.随着脂联素浓度升高,细胞凋亡率分别为（26.42±1.58）％、（15.14±1.91）％、（7.04±1.16）％、（5.05±0.83）％,均显著低于空白对照组细胞凋亡率[（33.18±1.86）％],差异有统计学意义（P＜0.05）.结论 脂联素抑制脂肪细胞凋亡是皮下缝合促进脂肪组织切口愈合的作用机制之一.Objective To investigate relationship between adiponectin and adipocyte apoptosis during adipose tissue wound healing （ATWH）,and clarify the role and its mechanism of adiponectin in subcutaneous suture to promote ATWH.Methods The pig models of ATWH were established to detect adiponectin expression of local adipose tissue by reverse transcriptase-polymerase chain reaction （RT-PCR）,and apoptosis was examined by fluorescent labeling technique.3T3-L1 preadipocytes of mice were cultured in vitro.In experimental group 3T3-L1 preadipocytes were cultured with different concentrations of adiponectin,and apoptosis rate was tested by Annexin-V-FITC method.Results The expression of adiponectin in subcutaneous suture was significantly higher than that in control group （P ＜ 0.05）.After 3 days and 5 days,the number of apoptotic cells was 29 ± 13 and 15 ± 8,respectively,statistically significantly less than that in the control group （69 ±25 and 23 ± 16） （P ＜0.05）.With adiponectin concentration rising,the number of apoptotic cells was （26.42 ± 1.58） ％,（15.14 ± 1.91） ％,（7.04 ± 1.16） ％ and （5.05 ± 0.83） ％,significantly less than that in control group （33.18 ± 1.86） ％ （P ＜ 0.05）.Conclusion Adiponectin plays a catalytic role in ATWH in the subcutaneous suture,and inhibiting adipocytes apoptosis is one of its mechanisms.

关 键 词：脂联素 皮下缝合 脱噬作用 切口愈合 

分 类 号：R363[医药卫生—病理学]