裸小鼠原位人膀胱癌细胞浅表膀胱癌模型的建立  

Establishment of the orthotopic superficial bladder cancer model device in human bladder cancer cell in nude mice

作　　者：李云龙[1] 严春寅[2] 李巧星[1] 吴刚 王伟录[1] 王勇[1] 郑红芳[1] 周洁[3] 

机构地区：[1]江苏大学附属昆山医院泌尿外科,江苏昆山215300 [2]苏州大学附属第一医院泌尿外科 [3]广西医科大学第一附属医院泌尿外科

出　　处：《中华实验外科杂志》2013年第12期2738-2740,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30700836）;江苏大学医学临床科技发展基金资助项目（JLY20120059）

摘　　要：目的 建立一种新型裸小鼠原位膀胱癌模型.方法 设计一种将人膀胱癌细胞种植在裸小鼠膀胱上的新型建模装置.采用24只BALB/c裸小鼠,建立裸小鼠原位人膀胱癌细胞浅表膀胱癌模型;随机分为两组,实验组应用本装置,经尿道插入裸小鼠膀胱,进行定位膀胱黏膜切割,再灌注EJ人膀胱癌细胞进行种植;对照组采用开腹后直视下用细穿刺针穿入膀胱腔内划割黏膜,再灌注EJ人膀胱癌细胞进行种植,监测两组膀胱癌细胞在裸小鼠膀胱黏膜上的生长.结果 成功地应用本模型建立装置建立裸小鼠膀胱癌原位模型,实验组成瘤率为91.7％ （11/12）,对照组成瘤率为55.6％ （5/9）,差异有统计学意义（P＜0.05）.结论 本膀胱癌原位模型的建立装置微创、简便、高效.Objective To explore a new type experimental research of orthotopic nude mouse model device in human bladder cancer cell.Methods A new modeling equipment to set up the orthotopic model device in human bladder cancer cell designed by myself was used in experimental group.Human bladder cancer cells were inoculated into 24 BALB/c nude mice.All these cases were divided equally into 2 groups：experimental group and control group.This device was used to insert nude mouse bladder transurethrally.This device was orientated to cut bladder mucosa,and human bladder cancer cell EJ was perfused for orthotopic xenotransplantion in nude mice by this model device.As to control group,bladder top was clamped,then pull out of incision to fix the bladder.With fine needle inserted in the bladder lumen cross cutting the bladder mucos in direct vision and reperfusion human bladder cancer cell EJ.And monitor two group membrane growth situation of orthotopic xenotransplanted tumor in nude mice.Results It was a success to use this new modeling equipment to set up the orthotopic model device in human bladder cancer cell.In experimental group,the tumor formation rate was 91.7％ （11/12） in experimental group.And the tumor formation rate was 55.6％ （5/9） in control group,which had obvious difference.Conclusion This new modeling equipment is a kind of mini-traumatic,simple and efficient tools to set up the orthotopic model device in human bladder cancer cell.

关 键 词：裸小鼠 膀胱癌 模型 动物 

分 类 号：R737.14[医药卫生—肿瘤]