鱼油对脂多糖刺激仔猪肠黏膜能量代谢的影响  

Effects of Fish Oil on Energy Metabolism in Intestinal Mucosa of Piglets after Lipopolysaccharide Challenge

作　　者：王秀英[1] 刘玉兰[1] 李藏兰[2] 陈逢[1] 朱惠玲[1] 侯永清[1] 丁斌鹰[1] 石海峰[1] 李爽[1] 任晓松 

机构地区：[1]武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉430023 [2]中国农业大学动物科技学院,北京100193 [3]福建高龙实业有限公司,福建福州350015

出　　处：《中国畜牧杂志》2013年第23期22-25,共4页Chinese Journal of Animal Science

基　　金：教育部新世纪优秀人才支持计划(NCET-10-0158);湖北省自然科学基金重点项目(2010CDA050);湖北省国际科技合作项目(2011BFA008);武汉市科技计划项目(201171034320)

摘　　要：试验研究鱼油对脂多糖(LPS)刺激仔猪肠黏膜能量代谢的影响。选用32头(28±3)d(8.91±0.74)kg的杜洛克×长白×大白仔猪,采用2×2因子设计,主因子包括日粮处理(5%鱼油或5%玉米油)和免疫应激(注射LPS或生理盐水),试验期19 d。试验第19天,每日粮组一半的猪注射100μg/kg BW的LPS,另一半注射生理盐水作对照。注射LPS 4 h后屠宰仔猪,取小肠样品待测。结果表明:鱼油对肠黏膜能量代谢的影响与LPS刺激不存在显著的互作关系;LPS刺激导致结肠三磷酸腺苷(ATP)水平显著下降(P<0.05);鱼油显著提高了结肠三磷酸腺苷(ATP)、一磷酸腺苷(AMP)、腺苷酸池(TAN)水平(P<0.05),对结肠AMP/ATP比值有提高的趋势(P<0.10),鱼油也显著降低了结肠能荷(EC)水平(P<0.05);鱼油和LPS刺激对空肠和回肠能量代谢指标均无显著影响。以上研究结果显示,鱼油可以缓解LPS应激引起的仔猪结肠黏膜能量代谢障碍,从而保护肠黏膜屏障。This experiment was conducted to investigate the effects of fish oil on energy metabolism in intestinal mucosa of piglets after lipopolysaccharide （LPS） challenge. Thirty-two crossbred （Duroc xLarge White xLandrace） piglets （8.91±0.74 kg BW, weaned at 28±3 d of age） were used to investigate in a 2×2 factorial design. The main factors consisted of diet （5% corn oil or 5% fish oil） and immunological challenge （LPS or saline）. On d 19, pigs were injected intraperitoneally with either 100 μg/kg body weight of LPS or an equivalent amount of sterile saline. At 4 h following LPS challenge, the pigs were slaughtered to collect intestinal mucosa for analysis. There was no LPS challenge x diet interaction observed for energy metabolism in intestinal mucosa. LPS reduced ATP level in colon （P〈 0.05）. Fish oil supplementation increased the levels of ATP, AMP and total adenine nucleotide in colon （P〈0.05）. Fish oil supplementation increased AMP/ATP ratio in colon （P〈0.10）. Fish oil supplementation reduced the levels of energy charge in colon （P〈0.05）. Both fish oil and LPS had no effects on energy metabolism in jejunum and ileum. These results suggest that fish oil alleviates the dysfunction of energy therefore exerts protective effects on intestinal mucosal barrier. metabolism in colon mucosa induced by LPS challenge,

关 键 词：鱼油 脂多糖 肠黏膜 能量代谢 仔猪 

分 类 号：S828.5[农业科学—畜牧学]