刀孢轮枝菌产红色素条件及稳定性研究  

Study of Producing Condition and the Stability of a Red Pigment from Lecanicillium psalliotae Zare & W. Gams

作　　者：王博[1] 徐龙光[1] 余仲东[1] 唐光辉[1] 陈辉[1] 

机构地区：[1]西北农林科技大学林学院,陕西杨陵712100

出　　处：《西北林学院学报》2013年第6期112-116,共5页Journal of Northwest Forestry University

基　　金：国家自然科学基金(31270690);长江学者和创新团队发展计划资助(IRT1035);校青年基本科研业务费(Z109021005)

摘　　要：分离了一株在PDA上强烈产生红色素的小叶杨(Populus simonii)内生菌,ITS-nrDNA序列分析及形态学初步鉴定为:刀孢轮枝菌(Lecanicillium psalliotae)。其产红色素是一种水溶性色素,在513nm处有强吸收峰,该色素在以葡萄糖为碳源的培养基中生长迅速,培养至23d色素达到最大的浓度2.73mg·mL-1,随后浓度逐渐下降。氧化剂H2O2和还原剂Na2SO3浓度逐步增大对色素有减色反应,色素在中性和碱性条件下红色加深,紫外光下长时间放置无大变化,但在紫外光照射3h时色素达到最大色度,温度对色素稳定性的影响较小。金属离子Ca2+、Fe2+、Cu2+和Zn2+对色素影响较大,能改变色素颜色。该色素浓度为2.11mg·mL-1时,对Escherichia coli和Pseudomonas syringa pv.actinidia拮抗作用明显。One fungus which secrets a red pigment was isolated from Populus simonii Carr. From the ITS-nrDNA phylogeny and morphology, it was identified as Lecanicillium psalliotae Zare ＆ W. Gams. The pigment was found to be water soluble, and presented strong absorbance at 513 nm.It could be produced rapidly in the carbon medium of glucose, and reached its maximum concentration of 2.73 mg·mL-1 after 23-day culturing,then concentration was down gradually. H2O2 and Na2SO3 both acted as reversed role. Under neutral and alkali solution, the red pigment would increase its redness and without big changes after placed in UV-light for a long time. Moreover, the red pigment achieved its maximum chromaticity at 3 h of UV exposure. The resorting temperature had no significant effect on the stability of the pigment. In addition, metallic ions such as Ca2＋, Fe2＋, Cu2＋, and Zn2＋ showed strong roles on the stability of the red pigment, the ions even changed the color drastically. The pigment with a concentration of 2.11 mg·mL-1 showed strong inhibiting activity to Escherichia coli and Pseudomonas syringa pv. actinidia.

关 键 词：刀孢轮枝菌 红色素 稳定性 

分 类 号：S718.81[农业科学—林学]