肠道细菌对柚皮苷的代谢研究  

Metabolism of naringin produced by intestinal bacteria

作　　者：张蔚[1] 江曙[1] 钱大玮[1] 尚尔鑫[1] 钱叶飞[1] 任浩[1] 管汉亮[1] 段金廒[1] 

机构地区：[1]南京中医药大学,江苏省方剂高技术研究重点实验室,江苏南京210046

出　　处：《药学学报》2013年第12期1817-1822,共6页Acta Pharmaceutica Sinica

基　　金：江苏省高校自然科学基金重大研究项目(10KJA360039);江苏省方剂高技术研究重点实验室建设项目(BM2010576)

摘　　要：研究人体肠道细菌对柚皮苷的代谢。采集健康志愿者新鲜粪便,称重稀释后厌氧培养,通过反复涂布分离得到69种人体肠道细菌。采用UPLC-Q-TOF/MS研究柚皮苷经粪便混合菌及分离的单菌作用后的代谢产物,共鉴定出乙酰化结合产物、苷元及氢化的苷元3种代谢产物;其中对柚皮苷有特殊代谢活性的4种单菌采用16S rRNA进行了鉴定,分别为Enterococcus sp.30、Bacillus sp.46、Escherichia sp.54和Escherichia sp.63。结果显示肠道细菌对柚皮苷有代谢作用且由不同细菌完成。Naringin has been reported to possess a wild range of biological activities. However, the route and metabolites of naringin produced by intestinal bacteria are not well understood. In this paper, different bacteria were isolated from human feces and their abilities to convert naringin to different metabolites were studied. Ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry （UPLC-Q-TOF/MS） with automated data analysis software （MetaboLynxTM） was applied to fast analysis of naringin metabolites. Using MSE and mass defect filter techniques, three metabolites were detected and tentatively identified. The results indicated that acetylation, hydrolyzation and hydrolyzation with hydrogenation were the major metabolic pathways of naringin/n vitro. Then, we studied the gene sequence of the 16S rRNA of the bacteria by extraction of genomic DNA of the strain, PCR amplification and clone of the 16S rRNA. The consequence proved that Enterococcus sp.30, Bacillus sp.46, Escherichia sp.54 and Escherich＆ sp.63 have the peculiar metabolism characteristic of naringin.

关 键 词：柚皮苷 肠道细菌 UPLC—Q—TOF MS 代谢产物 16S RRNA 

分 类 号：R917[医药卫生—药物分析学]